7XPX
Cryo-EM structure of the histone methyltransferase SET8 bound to H4K20Ecx-nucleosome
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB | Xenopus laevis (African clawed frog) | |
2 | B, F | Histone H4 | polymer | 102 | 11265.2 | 2 | Pfam (PF15511) UniProt (by SIFTS) (P62799) In PDB | Xenopus laevis | |
3 | C, G | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB | Xenopus laevis (African clawed frog) | |
4 | D, H | Histone H2B 1.1 | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
5 | I | DNA (145-MER) | polymer | 145 | 44761.5 | 1 | Homo sapiens | ||
6 | J | DNA (145-MER) | polymer | 145 | 44752.5 | 1 | Homo sapiens | ||
7 | K | N-lysine methyltransferase KMT5A | polymer | 221 | 25349.8 | 1 | UniProt (Q9NQR1) Pfam (PF00856) In PDB | Homo sapiens (human) | H4-K20-HMTase KMT5A,Histone-lysine N-methyltransferase KMT5A,Lysine N-methyltransferase 5A,Lysine-specific methylase 5A,PR/SET domain-containing protein 07,PR-Set7,PR/SET07,SET domain-containing protein 8 |
8 | K | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
K: 153 - 352 (UniProt: Q9NQR1)
PDB | External Database | Details |
---|---|---|
Thr 29 | Ser 33 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 132 | - | initiating methionine |
Gly 133 | - | expression tag |
Ser 134 | - | expression tag |
Ser 135 | - | expression tag |
His 136 | - | expression tag |
His 137 | - | expression tag |
His 138 | - | expression tag |
His 139 | - | expression tag |
His 140 | - | expression tag |
His 141 | - | expression tag |
Ser 142 | - | expression tag |
Ser 143 | - | expression tag |
Glu 144 | - | expression tag |
Asn 145 | - | expression tag |
Leu 146 | - | expression tag |
Tyr 147 | - | expression tag |
Phe 148 | - | expression tag |
Gln 149 | - | expression tag |
Gly 150 | - | expression tag |
His 151 | - | expression tag |
Met 152 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 223008.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 398.4 | |
All* | Total formula weight | 223406.6 |