7XPX
Cryo-EM structure of the histone methyltransferase SET8 bound to H4K20Ecx-nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11265.2 | 2 | Pfam (PF15511) UniProt (by SIFTS) (P62799) | Xenopus laevis | |
| 3 | C, G (C, G) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (145-MER) | polymer | 145 | 44761.5 | 1 | Homo sapiens | ||
| 6 | J (J) | DNA (145-MER) | polymer | 145 | 44752.5 | 1 | Homo sapiens | ||
| 7 | K (K) | N-lysine methyltransferase KMT5A | polymer | 221 | 25349.8 | 1 | UniProt (Q9NQR1) Pfam (PF00856) | Homo sapiens (human) | H4-K20-HMTase KMT5A,Histone-lysine N-methyltransferase KMT5A,Lysine N-methyltransferase 5A,Lysine-specific methylase 5A,PR/SET domain-containing protein 07,PR-Set7,PR/SET07,SET domain-containing protein 8 |
| 8 | L (K) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
K: 153 - 352 (UniProt: Q9NQR1)
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 132 | - | initiating methionine |
| Gly 133 | - | expression tag |
| Ser 134 | - | expression tag |
| Ser 135 | - | expression tag |
| His 136 | - | expression tag |
| His 137 | - | expression tag |
| His 138 | - | expression tag |
| His 139 | - | expression tag |
| His 140 | - | expression tag |
| His 141 | - | expression tag |
| Ser 142 | - | expression tag |
| Ser 143 | - | expression tag |
| Glu 144 | - | expression tag |
| Asn 145 | - | expression tag |
| Leu 146 | - | expression tag |
| Tyr 147 | - | expression tag |
| Phe 148 | - | expression tag |
| Gln 149 | - | expression tag |
| Gly 150 | - | expression tag |
| His 151 | - | expression tag |
| Met 152 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 223008.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 223406.6 |
