7XPX
Cryo-EM structure of the histone methyltransferase SET8 bound to H4K20Ecx-nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11265.2 | 2 | Pfam (PF15511) UniProt (by SIFTS) (P62799) | Xenopus laevis | |
| 3 | C, G (C, G) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (145-MER) | polymer | 145 | 44761.5 | 1 | Homo sapiens | ||
| 6 | J (J) | DNA (145-MER) | polymer | 145 | 44752.5 | 1 | Homo sapiens | ||
| 7 | K (K) | N-lysine methyltransferase KMT5A | polymer | 221 | 25349.8 | 1 | UniProt (Q9NQR1) Pfam (PF00856) | Homo sapiens (human) | H4-K20-HMTase KMT5A,Histone-lysine N-methyltransferase KMT5A,Lysine N-methyltransferase 5A,Lysine-specific methylase 5A,PR/SET domain-containing protein 07,PR-Set7,PR/SET07,SET domain-containing protein 8 |
| 8 | L (K) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
K: 153 - 352 (UniProt: Q9NQR1)
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 132 | - | initiating methionine |
| Gly 133 | - | expression tag |
| Ser 134 | - | expression tag |
| Ser 135 | - | expression tag |
| His 136 | - | expression tag |
| His 137 | - | expression tag |
| His 138 | - | expression tag |
| His 139 | - | expression tag |
| His 140 | - | expression tag |
| His 141 | - | expression tag |
| Ser 142 | - | expression tag |
| Ser 143 | - | expression tag |
| Glu 144 | - | expression tag |
| Asn 145 | - | expression tag |
| Leu 146 | - | expression tag |
| Tyr 147 | - | expression tag |
| Phe 148 | - | expression tag |
| Gln 149 | - | expression tag |
| Gly 150 | - | expression tag |
| His 151 | - | expression tag |
| Met 152 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 223008.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 223406.6 |
