7XPL

Crystal structure of a C/D-free RNA-guided RNA 2'-O-methyltransferase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	C/D box methylation guide ribonucleoprotein complex aNOP56 subunit	polymer	388	44168.5	2	UniProt (A0A0E3MJI1) Pfam (PF21572) Pfam (PF01798) UniProt (by SIFTS) (Q97ZH3)	Saccharolobus solfataricus	Pre mRNA splicing ribonucleoprotein,binding domain protein
2	C, D (E, F)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26455.4	2	UniProt (D0KTQ8) Pfam (PF01269) UniProt (by SIFTS) (P58032)	Saccharolobus solfataricus 98/2
3	E (G)	BMG3 RNA strand A	polymer	29	9252.5	1		Saccharolobus solfataricus
4	F (H)	BMG3 RNA strand B	polymer	29	9338.6	1		Saccharolobus solfataricus
5	G, H (I, J)	RNA (5'-R(*CP*CP*AP*UP*GP*AP*GP*UP*GP*UP*U)-3')	polymer	11	3484.1	2		Saccharolobus solfataricus
6	I, J (E, F)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
7	K, L, M, N, O... (A, B, E, F, G...)	water	water		18.0	253	Chemie (HOH)

Sequence modifications

A, B: 1 - 379 (UniProt: A0A0E3MJI1)

PDB	External Database	Details
Val 2	Met 2	conflict
Gln 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	166807.1
Non-Polymers*	Number of molecules	2
	Total formula weight	768.8
All*	Total formula weight	167576.0

*Water molecules are not included.