7XPL

Crystal structure of a C/D-free RNA-guided RNA 2'-O-methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	68.088, 76.185, 98.201
Unit cell angles	92.86, 94.08, 112.91

Refinement procedure

Resolution	41.705 - 2.213
R-factor	0.19
Rwork	0.189
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pla
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.134	1.136
Number of reflections	87481	4361
<I/σ(I)>	11.73
Completeness [%]	97.5	97.1
Redundancy	3.7	3.5
CC(1/2)		0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	291	0.2M sodium citrate tribasic dehydrate, 20% (w/v) polyethylene glycol 3350 (pH 8.3)