7XMT

CryoEM structure of somatostatin receptor 4 (SSTR4) with Gi1 and J-2156

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Somatostatin receptor type 4	polymer	375	40667.4	1	UniProt (P31391) Pfam (PF00001) In PDB	Homo sapiens (human)	SS-4-R,SS4-R,SS4R
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	S	single chain variable fragment of antibody	polymer	292	31549.1	1		Mus musculus
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
6	R	(2~{S})-2-[[(2~{S})-4-azanyl-2-[(4-methylnaphthalen-1-yl)sulfonylamino]butanoyl]amino]-3-phenyl-propanimidic acid	non-polymer		468.6	1	Chemie (I8B)

Sequence modifications

R: 2 - 328 (UniProt: P31391)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Phe 264	Val 264	conflict
Glu 329	-	expression tag
Phe 330	-	expression tag
Leu 331	-	expression tag
Glu 332	-	expression tag
Val 333	-	expression tag
Leu 334	-	expression tag
Phe 335	-	expression tag
Gln 336	-	expression tag
Gly 337	-	expression tag
Pro 338	-	expression tag
Trp 339	-	expression tag
Ser 340	-	expression tag
His 341	-	expression tag
Pro 342	-	expression tag
Gln 343	-	expression tag
Phe 344	-	expression tag
Glu 345	-	expression tag
Lys 346	-	expression tag
Gly 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Ser 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Gly 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Ser 357	-	expression tag
Ala 358	-	expression tag
Trp 359	-	expression tag
Ser 360	-	expression tag
His 361	-	expression tag
Pro 362	-	expression tag
Gln 363	-	expression tag
Phe 364	-	expression tag
Glu 365	-	expression tag
Lys 366	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	159269.1
Non-Polymers*	Number of molecules	1
	Total formula weight	468.6
All*	Total formula weight	159737.7

*Water molecules are not included.