7XMS

CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its endogeneous ligand SST-14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Somatostatin receptor type 4	polymer	375	40667.4	1	UniProt (P31391) Pfam (PF00001)	Homo sapiens (human)	SS-4-R,SS4-R,SS4R
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40403.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (S)	signle chain viable fragment of antibody	polymer	292	31549.1	1		Mus musculus
6	F (L)	Somatostatin-14	polymer	14	1641.9	1	UniProt (P61278) Pfam (PF03002)	Homo sapiens	SST14

Sequence modifications

R: 2 - 328 (UniProt: P31391)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Phe 264	Val 264	conflict
Glu 329	-	expression tag
Phe 330	-	expression tag
Leu 331	-	expression tag
Glu 332	-	expression tag
Val 333	-	expression tag
Leu 334	-	expression tag
Phe 335	-	expression tag
Gln 336	-	expression tag
Gly 337	-	expression tag
Pro 338	-	expression tag
Trp 339	-	expression tag
Ser 340	-	expression tag
His 341	-	expression tag
Pro 342	-	expression tag
Gln 343	-	expression tag
Phe 344	-	expression tag
Glu 345	-	expression tag
Lys 346	-	expression tag
Gly 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Ser 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Gly 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Ser 357	-	expression tag
Ala 358	-	expression tag
Trp 359	-	expression tag
Ser 360	-	expression tag
His 361	-	expression tag
Pro 362	-	expression tag
Gln 363	-	expression tag
Phe 364	-	expression tag
Glu 365	-	expression tag
Lys 366	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	160867.0
All*	Total formula weight	160867.0

*Water molecules are not included.