7XMR

CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its endogeneous peptide ligand SST-14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Somatostatin receptor type 2	polymer	406	45308.6	1	UniProt (P30874) Pfam (PF00001)	Homo sapiens (human)	SS-2-R,SS2-R,SS2R,SRIF-1
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40435.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (L)	Somatostatin-14	polymer	14	1641.9	1	UniProt (P61278) Pfam (PF03002)	Homo sapiens	SST14

Sequence modifications

R: 2 - 359 (UniProt: P30874)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Glu 360	-	expression tag
Phe 361	-	expression tag
Leu 362	-	expression tag
Glu 363	-	expression tag
Val 364	-	expression tag
Leu 365	-	expression tag
Phe 366	-	expression tag
Gln 367	-	expression tag
Gly 368	-	expression tag
Pro 369	-	expression tag
Trp 370	-	expression tag
Ser 371	-	expression tag
His 372	-	expression tag
Pro 373	-	expression tag
Gln 374	-	expression tag
Phe 375	-	expression tag
Glu 376	-	expression tag
Lys 377	-	expression tag
Gly 378	-	expression tag
Gly 379	-	expression tag
Gly 380	-	expression tag
Ser 381	-	expression tag
Gly 382	-	expression tag
Gly 383	-	expression tag
Gly 384	-	expression tag
Ser 385	-	expression tag
Gly 386	-	expression tag
Gly 387	-	expression tag
Ser 388	-	expression tag
Ala 389	-	expression tag
Trp 390	-	expression tag
Ser 391	-	expression tag
His 392	-	expression tag
Pro 393	-	expression tag
Gln 394	-	expression tag
Phe 395	-	expression tag
Glu 396	-	expression tag
Lys 397	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 55	Ile 55	conflict
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133991.1
All*	Total formula weight	133991.1

*Water molecules are not included.