7XCT

Cryo-EM structure of Dot1L and H2BK34ub-H3K79Nle nucleosome 2:1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, N (L, N)	Ubiquitin	polymer	76	8576.8	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
2	B, J (F, B)	Histone H4	polymer	89	10193.0	2	UniProt (A0A3P9NC92) Pfam (PF15511) UniProt (by SIFTS) (P62805)	Homo sapiens (human)
3	C, I (G, C)	Histone H2A	polymer	108	11865.9	2	UniProt (Q08AJ9) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04908)	Homo sapiens (human)
4	D, H (H, D)	Histone H2B type 1-K	polymer	93	10320.8	2	UniProt (O60814) Pfam (PF00125)	Homo sapiens (human)	H2B K,HIRA-interacting protein 1
5	E, M (K, M)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	328	37930.0	2	UniProt (Q8TEK3) Pfam (PF08123)	Homo sapiens (human)	DOT1-like protein
6	F, G (E, A)	Histone domain-containing protein	polymer	99	11615.6	2	UniProt (S4RAZ3) Pfam (PF00125) UniProt (by SIFTS) (Q71DI3)	Homo sapiens
7	K (I)	DNA (145-MER)	polymer	145	44521.4	1		synthetic construct
8	L (J)	DNA (145-MER)	polymer	145	44992.6	1		synthetic construct
9	O, P (K, M)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)

Sequence modifications

F, B: 15 - 102 (UniProt: A0A3P9NC92)

PDB	External Database	Details
Met 14	-	initiating methionine

E, A: 37 - 135 (UniProt: S4RAZ3)

PDB	External Database	Details
Nle 79	Lys 103	conflict

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	270518.4
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	271315.2

*Water molecules are not included.