7XCT

Cryo-EM structure of Dot1L and H2BK34ub-H3K79Nle nucleosome 2:1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, N (L, N)	Ubiquitin	polymer	76	8576.8	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
2	B, J (F, B)	Histone H4	polymer	89	10193.0	2	UniProt (A0A3P9NC92) Pfam (PF15511) UniProt (by SIFTS) (P62805)	Homo sapiens (human)
3	C, I (G, C)	Histone H2A	polymer	108	11865.9	2	UniProt (Q08AJ9) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04908)	Homo sapiens (human)
4	D, H (H, D)	Histone H2B type 1-K	polymer	93	10320.8	2	UniProt (O60814) Pfam (PF00125)	Homo sapiens (human)	H2B K,HIRA-interacting protein 1
5	E, M (K, M)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	328	37930.0	2	UniProt (Q8TEK3) Pfam (PF08123)	Homo sapiens (human)	DOT1-like protein
6	F, G (E, A)	Histone domain-containing protein	polymer	99	11615.6	2	UniProt (S4RAZ3) Pfam (PF00125) UniProt (by SIFTS) (Q71DI3)	Homo sapiens
7	K (I)	DNA (145-MER)	polymer	145	44521.4	1		synthetic construct
8	L (J)	DNA (145-MER)	polymer	145	44992.6	1		synthetic construct
9	O, P (K, M)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)

Sequence modifications

F, B: 15 - 102 (UniProt: A0A3P9NC92)

PDB	External Database	Details
Met 14	-	initiating methionine

E, A: 37 - 135 (UniProt: S4RAZ3)

PDB	External Database	Details
Nle 79	Lys 103	conflict

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	270518.4
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	271315.2

*Water molecules are not included.