7XBW

Cryo-EM structure of the human chemokine receptor CX3CR1 in complex with Gi1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	D	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38245.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	E	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	R	CX3C chemokine receptor 1	polymer	361	41072.7	1	UniProt (P49238) Pfam (PF00001) In PDB	Homo sapiens (human)	C-X3-C CKR-1,CX3CR1,Beta chemokine receptor-like 1,CMK-BRL-1,CMK-BRL1,Fractalkine receptor,G-protein coupled receptor 13,V28
5	R	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)

Sequence modifications

C: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

D: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

R: 1 - 315 (UniProt: P49238)

PDB	External Database	Details
Leu 120	Ile 120	engineered mutation
Ser 221	Cys 221	engineered mutation
Val 250	Met 250	engineered mutation
Glu 316	-	expression tag
Phe 317	-	expression tag
Leu 318	-	expression tag
Glu 319	-	expression tag
Val 320	-	expression tag
Leu 321	-	expression tag
Phe 322	-	expression tag
Gln 323	-	expression tag
Gly 324	-	expression tag
Pro 325	-	expression tag
Trp 326	-	expression tag
Ser 327	-	expression tag
His 328	-	expression tag
Pro 329	-	expression tag
Gln 330	-	expression tag
Phe 331	-	expression tag
Glu 332	-	expression tag
Lys 333	-	expression tag
Gly 334	-	expression tag
Gly 335	-	expression tag
Gly 336	-	expression tag
Ser 337	-	expression tag
Gly 338	-	expression tag
Gly 339	-	expression tag
Gly 340	-	expression tag
Ser 341	-	expression tag
Gly 342	-	expression tag
Gly 343	-	expression tag
Ser 344	-	expression tag
Ala 345	-	expression tag
Trp 346	-	expression tag
Ser 347	-	expression tag
His 348	-	expression tag
Pro 349	-	expression tag
Gln 350	-	expression tag
Phe 351	-	expression tag
Glu 352	-	expression tag
Lys 353	-	expression tag
Asp 354	-	expression tag
Tyr 355	-	expression tag
Lys 356	-	expression tag
Asp 357	-	expression tag
Asp 358	-	expression tag
Asp 359	-	expression tag
Asp 360	-	expression tag
Lys 361	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127626.8
Non-Polymers*	Number of molecules	3
	Total formula weight	1160.0
All*	Total formula weight	128786.7

*Water molecules are not included.