7X9B

Cryo-EM structure of neuropeptide Y Y2 receptor in complex with NPY and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Neuropeptide Y receptor type 2	polymer	391	43737.9	1	UniProt (P49146) Pfam (PF00001)	Homo sapiens (human)	NPY2-R,NPY-Y2 receptor,Y2 receptor
5	E (P)	Neuropeptide Y	polymer	36	4277.7	1	UniProt (P01303) Pfam (PF00159)	Homo sapiens	Neuropeptide tyrosine,NPY

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 1 - 353 (UniProt: P49146)

PDB	External Database	Details
Glu 354	-	expression tag
Phe 355	-	expression tag
Leu 356	-	expression tag
Glu 357	-	expression tag
Val 358	-	expression tag
Leu 359	-	expression tag
Phe 360	-	expression tag
Gln 361	-	expression tag
Gly 362	-	expression tag
Pro 363	-	expression tag
Trp 364	-	expression tag
Ser 365	-	expression tag
His 366	-	expression tag
Pro 367	-	expression tag
Gln 368	-	expression tag
Phe 369	-	expression tag
Glu 370	-	expression tag
Lys 371	-	expression tag
Gly 372	-	expression tag
Gly 373	-	expression tag
Gly 374	-	expression tag
Ser 375	-	expression tag
Gly 376	-	expression tag
Gly 377	-	expression tag
Gly 378	-	expression tag
Ser 379	-	expression tag
Gly 380	-	expression tag
Gly 381	-	expression tag
Ser 382	-	expression tag
Ala 383	-	expression tag
Trp 384	-	expression tag
Ser 385	-	expression tag
His 386	-	expression tag
Pro 387	-	expression tag
Gln 388	-	expression tag
Phe 389	-	expression tag
Glu 390	-	expression tag
Lys 391	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	135035.2
All*	Total formula weight	135035.2

*Water molecules are not included.