7X8S

Cryo-EM structure of the WB4-24-bound hGLP-1R-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) UniProt (by SIFTS) (P04896)	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody-35	polymer	140	15343.0	1		synthetic construct
5	E (R)	Glucagon-like peptide 1 receptor	polymer	440	50894.8	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	GLP-1 receptor,GLP-1-R,GLP-1R
6	F (R)	2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-(2-methylpropanoylamino)phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid	non-polymer		1017.1	1	Chemie (WB2)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	variant
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 24 - 463 (UniProt: P43220)

PDB	External Database	Details
Phe 260	Leu 260	variant

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	157566.7
Non-Polymers*	Number of molecules	1
	Total formula weight	1017.1
All*	Total formula weight	158583.8

*Water molecules are not included.