7X3T

Cryo-EM structure of ISW1a-dinucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, L, P (A, E, K, O)	Histone H3	polymer	136	15435.1	4	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233)	Xenopus laevis (African clawed frog)
2	B, F, M, Q (B, F, L, P)	Histone H4	polymer	103	11394.4	4	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G, N, R (C, G, M, Q)	Histone H2A	polymer	130	14109.4	4	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H, O, S (D, H, N, R)	Histone H2B 1.1	polymer	126	13965.3	4	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (343-MER)	polymer	354	108890.1	1		synthetic construct
6	J (J)	DNA(343-MER)	polymer	354	109775.7	1		synthetic construct
7	K (U)	ISWI one complex protein 3	polymer	624	72422.5	1	UniProt (P43596) Pfam (PF15612)	Saccharomyces cerevisiae S288C
8	T (V)	ISWI chromatin-remodeling complex ATPase ISW1	polymer	1062	123301.7	1	UniProt (P38144) Pfam (PF09111) Pfam (PF00176) Pfam (PF00271) Pfam (PF09110)	Saccharomyces cerevisiae S288C
9	U (V)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
10	V (V)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)
11	W (V)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

U: 127 - 749 (UniProt: P43596)

PDB	External Database	Details
Met 126	-	initiating methionine

V: 69 - 1129 (UniProt: P38144)

PDB	External Database	Details
Met 68	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	634007.0
Non-Polymers*	Number of molecules	3
	Total formula weight	517.5
All*	Total formula weight	634524.6

*Water molecules are not included.