7X2D
Cryo-EM structure of the tavapadon-bound D1 dopamine receptor and mini-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | F | D(1A) dopamine receptor | polymer | 473 | 52409.7 | 1 | UniProt (P21728) Pfam (PF00001) In PDB | Homo sapiens (human) | Dopamine D1 receptor |
2 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 248 | 28907.7 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB | Homo sapiens | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
4 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
5 | E | Nanobody35 | polymer | 160 | 17352.5 | 1 | synthetic construct | ||
6 | F | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
7 | F | 1,5-dimethyl-6-[2-methyl-4-[3-(trifluoromethyl)pyridin-2-yl]oxy-phenyl]pyrimidine-2,4-dione | non-polymer | 391.3 | 1 | Chemie (86W) |
Sequence modifications
F: 1 - 446 (UniProt: P21728)
B: 2 - 340 (UniProt: P62873)
D: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Met -26 | - | initiating methionine |
Lys -25 | - | expression tag |
Thr -24 | - | expression tag |
Ile -23 | - | expression tag |
Ile -22 | - | expression tag |
Ala -21 | - | expression tag |
Leu -20 | - | expression tag |
Ser -19 | - | expression tag |
Tyr -18 | - | expression tag |
Ile -17 | - | expression tag |
Phe -16 | - | expression tag |
Cys -15 | - | expression tag |
Leu -14 | - | expression tag |
Val -13 | - | expression tag |
Phe -12 | - | expression tag |
Ala -11 | - | expression tag |
Asp -10 | - | expression tag |
Tyr -9 | - | expression tag |
Lys -8 | - | expression tag |
Asp -7 | - | expression tag |
Asp -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Ala -3 | - | expression tag |
Ser -2 | - | expression tag |
Ile -1 | - | expression tag |
Asp 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -17 | - | initiating methionine |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 145933.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 778.0 | |
All* | Total formula weight | 146711.0 |