7X0B

The structure of a Twitch Radical SAM Dehydrogenase SpeY

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	4Fe-4S cluster-binding domain-containing protein	polymer	322	36014.6	2	UniProt (A8WEZ7) Pfam (PF04055)	Streptomyces spectabilis	SpeY
2	C, D, Q, R (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
3	E, S (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
4	F, G, H, I, T... (A, B)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
5	J, K, L, M, N... (A, B)	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
6	O, P (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	Y (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	AA, Z (B, A)	water	water		18.0	265	Chemie (HOH)

Sequence modifications

A, B: 1 - 302 (UniProt: A8WEZ7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 269	Glu 269	conflict
Ala 270	Glu 270	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72029.3
Non-Polymers*	Number of molecules	23
	Total formula weight	3368.0
All*	Total formula weight	75397.3

*Water molecules are not included.