7WZV

The structure of a Twitch Radical SAM Dehydrogenase SpeY

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	4Fe-4S cluster-binding domain-containing protein	polymer	322	36014.6	2	UniProt (A8WEZ7) Pfam (PF04055) In PDB	Streptomyces spectabilis	SpeY
2	A, B	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
3	A, B	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
4	A, B	(1~{S},2~{R},4~{S},5~{R})-2,4-bis(methylamino)-6-[(2~{S},3~{R},4~{S},6~{R})-6-methyl-3,4-bis(oxidanyl)oxan-2-yl]oxy-cyclohexane-1,3,5-triol	non-polymer		336.4	2	Chemie (7P8)
5	B, A	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
6	B, A	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
7	B, A	GLYCEROL	non-polymer		92.1	11	Chemie (GOL)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
10		water	water		18.0	357	Chemie (HOH)

Sequence modifications

A, B: 1 - 302 (UniProt: A8WEZ7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 269	Glu 269	conflict
Ala 270	Glu 270	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72029.3
Non-Polymers*	Number of molecules	33
	Total formula weight	4774.1
All*	Total formula weight	76803.4

*Water molecules are not included.