7WVY

Cryo-EM structure of the human formyl peptide receptor 2 in complex with Abeta42 and Gi2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Amyloid-beta A4 protein	polymer	42	4520.1	1	UniProt (B4DMD5) Pfam (PF03494) UniProt (by SIFTS) (P05067)	Homo sapiens
2	B (R)	Soluble cytochrome b562,N-formyl peptide receptor 2	polymer	513	56710.3	1	UniProt (P0ABE7) UniProt (P25090) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562,FMLP-related receptor I,FMLP-R-I,Formyl peptide receptor-like 1,HM63,Lipoxin A4 receptor,LXA4 receptor,RFP
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I

Sequence modifications

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Gly -118	-	expression tag
Ala -117	-	expression tag
Pro -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Gly -9	-	linker
Ser -8	-	linker
Gly -7	-	linker
Ser -6	-	linker
Glu -5	-	linker
Asn -4	-	linker
Leu -3	-	linker
Tyr -2	-	linker
Phe -1	-	linker
Gln 0	-	linker
Ser 1	-	linker

R: 2 - 347 (UniProt: P25090)

PDB	External Database	Details
Leu 211	Ser 211	engineered mutation
Phe 348	-	expression tag
Leu 349	-	expression tag
Glu 350	-	expression tag
Val 351	-	expression tag
Leu 352	-	expression tag
Phe 353	-	expression tag
Gln 354	-	expression tag
Gly 355	-	expression tag
Pro 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Trp 359	-	expression tag
Ser 360	-	expression tag
His 361	-	expression tag
Pro 362	-	expression tag
Gln 363	-	expression tag
Phe 364	-	expression tag
Glu 365	-	expression tag
Lys 366	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Ala 370	-	expression tag
Gly 371	-	expression tag
Ala 372	-	expression tag
Ser 373	-	expression tag
Ala 374	-	expression tag
Gly 375	-	expression tag
Ser 376	-	expression tag
Trp 377	-	expression tag
Ser 378	-	expression tag
His 379	-	expression tag
Pro 380	-	expression tag
Gln 381	-	expression tag
Phe 382	-	expression tag
Glu 383	-	expression tag
Lys 384	-	expression tag
Gly 385	-	expression tag
Ser 386	-	expression tag
Asp 387	-	expression tag
Tyr 388	-	expression tag
Lys 389	-	expression tag
Asp 390	-	expression tag
Asp 391	-	expression tag
Asp 392	-	expression tag
Asp 393	-	expression tag
Lys 394	-	expression tag

A: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Ser 327	Ala 327	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148338.8
All*	Total formula weight	148338.8

*Water molecules are not included.