7WUQ

Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody-35	polymer	128	13885.4	1		Lama glama
5	R	Adhesion G-protein coupled receptor G2,mCherry	polymer	683	77147.9	1	UniProt (Q8CJ12) Pfam (PF00002) In PDB	Mus musculus (house mouse)	G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 597 - 1009 (UniProt: Q8CJ12)

PDB	External Database	Details
Met 581	-	expression tag
Lys 582	-	expression tag
Thr 583	-	expression tag
Ile 584	-	expression tag
Ile 585	-	expression tag
Ala 586	-	expression tag
Leu 587	-	expression tag
Ser 588	-	expression tag
Tyr 589	-	expression tag
Ile 590	-	expression tag
Phe 591	-	expression tag
Cys 592	-	expression tag
Leu 593	-	expression tag
Val 594	-	expression tag
Phe 595	-	expression tag
Ala 596	-	expression tag
His 1010	-	linker
His 1011	-	linker
His 1012	-	linker
His 1013	-	linker
His 1014	-	linker
His 1015	-	linker
His 1016	-	linker
His 1017	-	linker
Gly 1018	-	linker
Ser 1019	-	linker
Ala 1020	-	linker
Glu 1021	-	linker
Asn 1022	-	linker
Leu 1023	-	linker
Tyr 1024	-	linker
Phe 1025	-	linker
Gln 1026	-	linker
Gly 1027	-	linker

R: 1028 - 1263 (PDB: 7WUQ)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184067.1
All*	Total formula weight	184067.1

*Water molecules are not included.