7WU9
Cryo-EM structure of the human EP3-Gi signaling complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | R | Prostaglandin E2 receptor EP3 subtype | polymer | 325 | 36175.9 | 1 | UniProt (P43115) Pfam (PF00001) In PDB | Homo sapiens (human) | PGE receptor EP3 subtype,PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor |
| 2 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 355 | 40428.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
| 5 | S | scFv16 | polymer | 255 | 27318.4 | 1 | Mus musculus (Mouse) |
Sequence modifications
R: 47 - 359 (UniProt: P43115)
A: 2 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Gly 43 | - | expression tag |
| Pro 44 | - | expression tag |
| Thr 45 | - | expression tag |
| Ser 46 | - | expression tag |
| Ile 173 | Ala 173 | engineered mutation |
| Ser 185 | Val 185 | engineered mutation |
| Gln 217 | Asn 217 | engineered mutation |
| Asp 258 | Ser 258 | engineered mutation |
| Leu 289 | Cys 289 | engineered mutation |
| Gln 308 | Asn 308 | engineered mutation |
| Leu 360 | - | expression tag |
| Glu 361 | - | expression tag |
| Leu 362 | - | expression tag |
| Glu 363 | - | expression tag |
| Val 364 | - | expression tag |
| Leu 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Gln 367 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 149511.5 | |
| All* | Total formula weight | 149511.5 |
