7WU5

Cryo-EM structure of the adhesion GPCR ADGRF1(H565A/T567A) in complex with miniGi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	241	27563.4	1	UniProt (A0A3B3IUA8) UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Adhesion G-protein coupled receptor F1	polymer	674	74513.4	1	UniProt (Q5T601) Pfam (PF01825) Pfam (PF00002)	Homo sapiens (human)	G protein-coupled receptor 110,G protein-coupled receptor KPG_012,G protein-coupled receptor PGR19
5	E (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
6	F (R)	[(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate	non-polymer		496.6	1	Chemie (K6G)

Sequence modifications

A: 4 - 172 (UniProt: A0A3B3IUA8)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict
Gly 173	-	linker
Gly 174	-	linker
Gly 175	-	linker
Gly 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Gly 179	-	linker
Gly 180	-	linker

A: 181 - 354 (UniProt: P63096)

PDB	External Database	Details
Asp 217	Gly 217	conflict
Ala 219	Thr 219	conflict
Asp 226	Ala 226	conflict
-	Asp 231	deletion
-	Leu 232	deletion
-	Val 233	deletion
-	Leu 234	deletion
-	Ala 235	deletion
-	Glu 236	deletion
-	Asp 237	deletion
-	Glu 238	deletion
-	Glu 239	deletion
-	Met 240	deletion
Gln 288	Pro 288	conflict
Ala 332	Val 332	conflict
Ile 335	Val 335	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 251 - 860 (UniProt: Q5T601)

PDB	External Database	Details
Met 225	-	initiating methionine
Lys 226	-	expression tag
Thr 227	-	expression tag
Ile 228	-	expression tag
Ile 229	-	expression tag
Ala 230	-	expression tag
Leu 231	-	expression tag
Ser 232	-	expression tag
Tyr 233	-	expression tag
Ile 234	-	expression tag
Phe 235	-	expression tag
Cys 236	-	expression tag
Leu 237	-	expression tag
Val 238	-	expression tag
Phe 239	-	expression tag
Ala 240	-	expression tag
Asp 241	-	expression tag
Tyr 242	-	expression tag
Lys 243	-	expression tag
Asp 244	-	expression tag
Asp 245	-	expression tag
Asp 246	-	expression tag
Asp 247	-	expression tag
Gly 248	-	expression tag
Ala 249	-	expression tag
Pro 250	-	expression tag
Ala 565	His 565	engineered mutation
Ala 567	Thr 567	engineered mutation
Glu 861	-	expression tag
Phe 862	-	expression tag
Leu 863	-	expression tag
Glu 864	-	expression tag
Val 865	-	expression tag
Leu 866	-	expression tag
Phe 867	-	expression tag
Gln 868	-	expression tag
Gly 869	-	expression tag
Pro 870	-	expression tag
Trp 871	-	expression tag
Ser 872	-	expression tag
His 873	-	expression tag
Pro 874	-	expression tag
Gln 875	-	expression tag
Phe 876	-	expression tag
Glu 877	-	expression tag
Lys 878	-	expression tag
Gly 879	-	expression tag
Gly 880	-	expression tag
Gly 881	-	expression tag
Ser 882	-	expression tag
Gly 883	-	expression tag
Gly 884	-	expression tag
Gly 885	-	expression tag
Ser 886	-	expression tag
Gly 887	-	expression tag
Gly 888	-	expression tag
Ser 889	-	expression tag
Ala 890	-	expression tag
Trp 891	-	expression tag
Ser 892	-	expression tag
His 893	-	expression tag
Pro 894	-	expression tag
Gln 895	-	expression tag
Phe 896	-	expression tag
Glu 897	-	expression tag
Lys 898	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148682.3
Non-Polymers*	Number of molecules	2
	Total formula weight	883.3
All*	Total formula weight	149565.6

*Water molecules are not included.