7WU4

Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	241	27563.4	1	UniProt (A0A3B3IUA8) UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	R	Adhesion G-protein coupled receptor F1	polymer	674	74610.5	1	UniProt (Q5T601) Pfam (PF01825) Pfam (PF00002) In PDB	Homo sapiens (human)	G protein-coupled receptor 110,G protein-coupled receptor KPG_012,G protein-coupled receptor PGR19
5	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
6	R	[(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate	non-polymer		496.6	1	Chemie (K6G)

Sequence modifications

A: 4 - 172 (UniProt: A0A3B3IUA8)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict
Gly 173	-	linker
Gly 174	-	linker
Gly 175	-	linker
Gly 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Gly 179	-	linker
Gly 180	-	linker

A: 181 - 354 (UniProt: P63096)

PDB	External Database	Details
Asp 217	Gly 217	conflict
Ala 219	Thr 219	conflict
Asp 226	Ala 226	conflict
-	Asp 231	deletion
-	Leu 232	deletion
-	Val 233	deletion
-	Leu 234	deletion
-	Ala 235	deletion
-	Glu 236	deletion
-	Asp 237	deletion
-	Glu 238	deletion
-	Glu 239	deletion
-	Met 240	deletion
Gln 288	Pro 288	conflict
Ala 332	Val 332	conflict
Ile 335	Val 335	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 251 - 860 (UniProt: Q5T601)

PDB	External Database	Details
Met 225	-	initiating methionine
Lys 226	-	expression tag
Thr 227	-	expression tag
Ile 228	-	expression tag
Ile 229	-	expression tag
Ala 230	-	expression tag
Leu 231	-	expression tag
Ser 232	-	expression tag
Tyr 233	-	expression tag
Ile 234	-	expression tag
Phe 235	-	expression tag
Cys 236	-	expression tag
Leu 237	-	expression tag
Val 238	-	expression tag
Phe 239	-	expression tag
Ala 240	-	expression tag
Asp 241	-	expression tag
Tyr 242	-	expression tag
Lys 243	-	expression tag
Asp 244	-	expression tag
Asp 245	-	expression tag
Asp 246	-	expression tag
Asp 247	-	expression tag
Gly 248	-	expression tag
Ala 249	-	expression tag
Pro 250	-	expression tag
Glu 861	-	expression tag
Phe 862	-	expression tag
Leu 863	-	expression tag
Glu 864	-	expression tag
Val 865	-	expression tag
Leu 866	-	expression tag
Phe 867	-	expression tag
Gln 868	-	expression tag
Gly 869	-	expression tag
Pro 870	-	expression tag
Trp 871	-	expression tag
Ser 872	-	expression tag
His 873	-	expression tag
Pro 874	-	expression tag
Gln 875	-	expression tag
Phe 876	-	expression tag
Glu 877	-	expression tag
Lys 878	-	expression tag
Gly 879	-	expression tag
Gly 880	-	expression tag
Gly 881	-	expression tag
Ser 882	-	expression tag
Gly 883	-	expression tag
Gly 884	-	expression tag
Gly 885	-	expression tag
Ser 886	-	expression tag
Gly 887	-	expression tag
Gly 888	-	expression tag
Ser 889	-	expression tag
Ala 890	-	expression tag
Trp 891	-	expression tag
Ser 892	-	expression tag
His 893	-	expression tag
Pro 894	-	expression tag
Gln 895	-	expression tag
Phe 896	-	expression tag
Glu 897	-	expression tag
Lys 898	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148779.4
Non-Polymers*	Number of molecules	2
	Total formula weight	883.3
All*	Total formula weight	149662.7

*Water molecules are not included.