7WJU
Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | OrfB_Zn_ribbon domain-containing protein | polymer | 422 | 48719.7 | 2 | UniProt (A0A2U3D0N8) Pfam (PF07282) | Acidibacillus sulfuroxidans | |
| 2 | C (C) | sgRNAv1 | polymer | 191 | 61643.6 | 1 | synthetic construct | ||
| 3 | D (D) | Target strand | polymer | 30 | 9261.0 | 1 | synthetic construct | ||
| 4 | E (E) | Non-target strand | polymer | 6 | 1840.2 | 1 | synthetic construct |
Sequence modifications
A, B: 1 - 422 (UniProt: A0A2U3D0N8)
| PDB | External Database | Details |
|---|---|---|
| Ala 225 | Asp 225 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 170184.3 | |
| All* | Total formula weight | 170184.3 |
