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- EMDB-32548: Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex -
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Open data
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Basic information
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Title | Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex | ||||||||||||
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Function / homology | Transposase IS605, OrfB, C-terminal / Putative transposase DNA-binding domain / ![]() ![]() ![]() ![]() ![]() ![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | Wu Z / Liu D / Shen H / Ji Q | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure-directed functional evolution of the miniature CRISPR-AsCas12f1 system Authors: Wu Z / Liu D / Pan D / Shen H / Ji Q | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.1 KB 13.1 KB | Display Display | ![]() |
Images | ![]() | 81.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7wjuMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.0773 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : AsCas12f1-sgRNAv1-dsDNA ternary complex
Entire | Name: AsCas12f1-sgRNAv1-dsDNA ternary complex |
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Components |
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-Supramolecule #1: AsCas12f1-sgRNAv1-dsDNA ternary complex
Supramolecule | Name: AsCas12f1-sgRNAv1-dsDNA ternary complex / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Supramolecule #2: AsCas12f1
Supramolecule | Name: AsCas12f1 / type: complex / ID: 2 / Chimera: Yes / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: synthetic construct (others) |
-Supramolecule #3: sgRNA1
Supramolecule | Name: sgRNA1 / type: complex / ID: 3 / Chimera: Yes / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: synthetic construct (others) |
-Supramolecule #4: dsDNA
Supramolecule | Name: dsDNA / type: complex / ID: 4 / Chimera: Yes / Parent: 1 / Macromolecule list: #3-#4 |
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-Macromolecule #1: OrfB_Zn_ribbon domain-containing protein
Macromolecule | Name: OrfB_Zn_ribbon domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 48.719742 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MIKVYRYEIV KPLDLDWKEF GTILRQLQQE TRFALNKATQ LAWEWMGFSS DYKDNHGEYP KSKDILGYTN VHGYAYHTIK TKAYRLNSG NLSQTIKRAT DRFKAYQKEI LRGDMSIPSY KRDIPLDLIK ENISVNRMNH GDYIASLSLL SNPAKQEMNV K RKISVIII ...String: MIKVYRYEIV KPLDLDWKEF GTILRQLQQE TRFALNKATQ LAWEWMGFSS DYKDNHGEYP KSKDILGYTN VHGYAYHTIK TKAYRLNSG NLSQTIKRAT DRFKAYQKEI LRGDMSIPSY KRDIPLDLIK ENISVNRMNH GDYIASLSLL SNPAKQEMNV K RKISVIII VRGAGKTIMD RILSGEYQVS ASQIIHDDRK NKWYLNISYD FEPQTRVLDL NKIMGIALGV AVAVYMAFQH TP ARYKLEG GEIENFRRQV ESRRISMLRQ GKYAGGARGG HGRDKRIKPI EQLRDKIANF RDTTNHRYSR YIVDMAIKEG CGT IQMEDL TNIRDIGSRF LQNWTYYDLQ QKIIYKAEEA GIKVIKIDPQ YTSQRCSECG NIDSGNRIGQ AIFKCRACGY EANA DYNAA RNIAIPNIDK IIAESIK |
-Macromolecule #2: sgRNAv1
Macromolecule | Name: sgRNAv1 / type: rna / ID: 2 / Number of copies: 1 |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 61.643578 KDa |
Sequence | String: AUUCGUCGGU UCAGCGACGA UAAGCCGAGA AGUGCCAAUA AAACUGUUAA GUGGUUUGGU AACGCUCGGU AAGGUAGCCA AAAGGCUGA AACUCCGUGC ACAAAGACCG CACGGACGCU UCACAUAUAG CUCAUAAACA AGGGUUUGCG AGCUAGCUUG U GGAGUGUG AACCUGGCGU UUUUCCAUAG GCU |
-Macromolecule #3: Target strand
Macromolecule | Name: Target strand / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 9.260985 KDa |
Sequence | String: (DG)(DC)(DG)(DG)(DA)(DG)(DC)(DC)(DT)(DA) (DT)(DG)(DG)(DA)(DA)(DA)(DA)(DA)(DC)(DG) (DC)(DC)(DA)(DG)(DC)(DA)(DA)(DC)(DG) (DC) |
-Macromolecule #4: Non-target strand
Macromolecule | Name: Non-target strand / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 1.840227 KDa |
Sequence | String: (DG)(DC)(DG)(DT)(DT)(DG) |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
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Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.69 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1770878 |