7WJD

Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase mutant D394A in complex with nigerotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-xylosidase	polymer	759	87984.5	1	UniProt (A2RM80) Pfam (PF01055) Pfam (PF21365) In PDB	Lactococcus lactis subsp. cremoris MG1363
2	B	alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		504.4	1
3	A	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5		water	water		18.0	480	Chemie (HOH)

Sequence modifications

A: 1 - 739 (UniProt: A2RM80)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 394	Asp 394	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	87984.5
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	3
	Total formula weight	304.3
All*	Total formula weight	88793.2

*Water molecules are not included.