7WJD
Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase mutant D394A in complex with nigerotriose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha-xylosidase | polymer | 759 | 87984.5 | 1 | UniProt (A2RM80) Pfam (PF01055) Pfam (PF21365) | Lactococcus lactis subsp. cremoris MG1363 | |
| 2 | B (B) | alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose | branched | 504.4 | 1 | ||||
| 3 | C (A) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 4 | D, E (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 5 | F (A) | water | water | 18.0 | 480 | Chemie (HOH) |
Sequence modifications
A: 1 - 739 (UniProt: A2RM80)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 394 | Asp 394 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 87984.5 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 504.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 304.3 | |
| All* | Total formula weight | 88793.2 |
