7WJ5

Cryo-EM structure of human somatostatin receptor 2 complex with its agonist somatostatin delineates the ligand binding specificity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (E)	Gai1 antibody (scfv16)	polymer	266	28636.8	1		Homo sapiens
5	E (R)	Soluble cytochrome b562,Somatostatin receptor type 2	polymer	518	58163.5	1	UniProt (P0ABE7) UniProt (P30874) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,SS-2-R,SS2-R,SS2R,SRIF-1
6	F (S)	Somatostatin-14 (SST-14)	polymer	14	1641.9	1	Pfam (PF03002) UniProt (by SIFTS) (P61278) BIRD (PRD_001216)	Homo sapiens

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -105 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -148	-	initiating methionine
Lys -147	-	expression tag
Thr -146	-	expression tag
Ile -145	-	expression tag
Ile -144	-	expression tag
Ala -143	-	expression tag
Leu -142	-	expression tag
Ser -141	-	expression tag
Tyr -140	-	expression tag
Ile -139	-	expression tag
Phe -138	-	expression tag
Cys -137	-	expression tag
Leu -136	-	expression tag
Val -135	-	expression tag
Phe -134	-	expression tag
Ala -133	-	expression tag
Asp -132	-	expression tag
Tyr -131	-	expression tag
Lys -130	-	expression tag
Asp -129	-	expression tag
Asp -128	-	expression tag
Asp -127	-	expression tag
Asp -126	-	expression tag
Lys -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
Leu -116	-	expression tag
Glu -115	-	expression tag
Val -114	-	expression tag
Leu -113	-	expression tag
Phe -112	-	expression tag
Gln -111	-	expression tag
Gly -110	-	expression tag
Pro -109	-	expression tag
Gly -108	-	expression tag
Ala -107	-	expression tag
Pro -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	-	linker

R: 1 - 369 (UniProt: P30874)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	176136.4
All*	Total formula weight	176136.4

*Water molecules are not included.