7WIC
Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | L | somatostatin-14 | polymer | 14 | 1641.9 | 1 | Pfam (PF03002) UniProt (by SIFTS) (P61278) In PDB BIRD (PRD_001216) | Homo sapiens | |
| 2 | R | Somatostatin receptor type 2 | polymer | 369 | 41368.6 | 1 | UniProt (P30874) Pfam (PF00001) In PDB | Homo sapiens (human) | SS-2-R,SS2-R,SS2R,SRIF-1 |
| 3 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (cattle) | G gamma-I |
| 6 | S | single Fab chain (svFv16) | polymer | 269 | 28813.0 | 1 | synthetic construct |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 157883.1 | |
| All* | Total formula weight | 157883.1 |
