7WFC

X-ray structure of HKU1-PLP2(Cys109Ser) catalytic mutant in complex with free ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Papain-like protease	polymer	309	34550.7	1	UniProt (U3N841) Pfam (PF08715) In PDB	Human coronavirus HKU1
2	B	60S ribosomal protein L40	polymer	81	9048.4	1	UniProt (L9KW39) Pfam (PF00240) UniProt (by SIFTS) (P62987) In PDB	Homo sapiens (human)	CEP52,Ubiquitin,Ubiquitin A-52 residue ribosomal protein fusion product 1,Ubiquitin-60S ribosomal protein L40
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5		water	water		18.0	195	Chemie (HOH)

Sequence modifications

A: 3 - 311 (UniProt: U3N841)

PDB	External Database	Details
Ser 109	Cys 1742	engineered mutation

B: 1 - 76 (UniProt: L9KW39)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43599.1
Non-Polymers*	Number of molecules	2
	Total formula weight	127.5
All*	Total formula weight	43726.6

*Water molecules are not included.