7WF7

Cryo-EM of Sphingosine 1-phosphate receptor 1 / Gi complex bound to S1P

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sphingosine 1-phosphate receptor 1	polymer	382	42898.8	1	UniProt (P21453) Pfam (PF00001) In PDB	Homo sapiens (human)	S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1
2	B	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	scFv16	polymer	247	26293.3	1		Homo sapiens
6	A	(2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate	non-polymer		379.5	1	Chemie (S1P)

Sequence modifications

A: 1 - 382 (UniProt: P21453)

PDB	External Database	Details
Trp 133	Phe 133	conflict

B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	155413.8
Non-Polymers*	Number of molecules	1
	Total formula weight	379.5
All*	Total formula weight	155793.2

*Water molecules are not included.