7WCN
Cryo-EM structure of GPR119-Gs Complex with small molecule agonist AR231453
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
| 4 | N | Nb35 | polymer | 138 | 15140.7 | 1 | Lama glama | ||
| 5 | R | Glucose-dependent insulinotropic receptor | polymer | 335 | 36936.7 | 1 | UniProt (Q8TDV5) Pfam (PF00001) In PDB | Homo sapiens (human) | G-protein coupled receptor 119 |
| 6 | R | N-(2-fluoranyl-4-methylsulfonyl-phenyl)-5-nitro-6-[4-(3-propan-2-yl-1,2,4-oxadiazol-5-yl)piperidin-1-yl]pyrimidin-4-amine | non-polymer | 505.5 | 1 | Chemie (8WL) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 13 - 351 (UniProt: P62873)
R: 1 - 335 (UniProt: Q8TDV5)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Leu 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Gln 12 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 237 | Ser 237 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 144366.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 505.5 | |
| All* | Total formula weight | 144871.9 |
