7WAH

Structure of Cas7-11 in complex with guide RNA and target RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	crRNA (39-MER)	polymer	39	12502.4	1		Escherichia coli
2	B (D)	tgRNA (5'-R(P*UP*AP*CP*CP*CP*AP*UP*GP*UP*CP*GP*AP*AP*GP*AP*CP*AP*AP*CP*AP*AP*AP*G)-3')	polymer	23	7377.5	1		Escherichia coli
3	C (A)	CRISPR-associated RAMP family protein	polymer	1617	185609.9	1	UniProt (A0A401FT36) Pfam (PF03787)	Desulfonema ishimotonii
4	D, E, F, G (A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 1 - 1601 (UniProt: A0A401FT36)

PDB	External Database	Details
Ala 429	Asp 429	engineered mutation
Ala 654	Asp 654	engineered mutation
Ala 753	Asp 753	engineered mutation
Ser 1602	-	expression tag
Ser 1603	-	expression tag
Gly 1604	-	expression tag
Leu 1605	-	expression tag
Val 1606	-	expression tag
Pro 1607	-	expression tag
Arg 1608	-	expression tag
Gly 1609	-	expression tag
Ser 1610	-	expression tag
His 1611	-	expression tag
His 1612	-	expression tag
His 1613	-	expression tag
His 1614	-	expression tag
His 1615	-	expression tag
His 1616	-	expression tag
His 1617	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	205489.8
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	205751.4

*Water molecules are not included.