7W9T
Cryo-EM structure of human Nav1.7(E406K) in complex with auxiliary beta subunits, huwentoxin-IV and saxitoxin (S6IV alpha helix conformer)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sodium channel protein type 9 subunit alpha | polymer | 2031 | 231211.9 | 1 | UniProt (Q15858) Pfam (PF00520) Pfam (PF11933) Pfam (PF06512) Pfam (PF24609) | Homo sapiens (human) | Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7 |
| 2 | B (B) | Sodium channel subunit beta-1 | polymer | 218 | 24732.1 | 1 | UniProt (Q07699) Pfam (PF07686) | Homo sapiens (human) | Nav1.7 beta1 subunit |
| 3 | C (C) | Sodium channel subunit beta-2 | polymer | 215 | 24355.9 | 1 | UniProt (O60939) Pfam (PF07686) | Homo sapiens (human) | Nav1.7 beta2 subunit |
| 4 | D, E, F (D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 5 | G (A) | [(3aS,4R,10aS)-2,6-diamino-10,10-dihydroxy-3a,4,9,10-tetrahydro-3H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate | non-polymer | 299.3 | 1 | Chemie (9SL) | |||
| 6 | H, JA, KA, LA, N (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 7 | I, W (A) | O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine | non-polymer | 792.1 | 2 | Chemie (P5S) | |||
| 8 | IA, J, K, L, O (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 5 | Chemie (Y01) | |||
| 9 | M (A) | (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en | non-polymer | 544.8 | 1 | Chemie (9Z9) | |||
| 10 | AA, BA, CA, DA, EA... (A, B) | 1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 510.7 | 14 | Chemie (LPE) | |||
| 11 | GA, HA, S, V, X (A) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 5 | Chemie (PCW) |
Sequence modifications
A: 1 - 1988 (UniProt: Q15858)
| PDB | External Database | Details |
|---|---|---|
| Met -42 | - | expression tag |
| Ala -41 | - | expression tag |
| Ser -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Lys 406 | Glu 406 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 280299.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 17053.4 | |
| All* | Total formula weight | 298626.5 |
