7W9T
Cryo-EM structure of human Nav1.7(E406K) in complex with auxiliary beta subunits, huwentoxin-IV and saxitoxin (S6IV alpha helix conformer)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sodium channel protein type 9 subunit alpha | polymer | 2031 | 231211.9 | 1 | UniProt (Q15858) Pfam (PF00520) Pfam (PF11933) Pfam (PF06512) Pfam (PF24609) | Homo sapiens (human) | Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7 |
| 2 | B (B) | Sodium channel subunit beta-1 | polymer | 218 | 24732.1 | 1 | UniProt (Q07699) Pfam (PF07686) | Homo sapiens (human) | Nav1.7 beta1 subunit |
| 3 | C (C) | Sodium channel subunit beta-2 | polymer | 215 | 24355.9 | 1 | UniProt (O60939) Pfam (PF07686) | Homo sapiens (human) | Nav1.7 beta2 subunit |
| 4 | D, E, F (D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 5 | G (A) | [(3aS,4R,10aS)-2,6-diamino-10,10-dihydroxy-3a,4,9,10-tetrahydro-3H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate | non-polymer | 299.3 | 1 | Chemie (9SL) | |||
| 6 | H, JA, KA, LA, N (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 7 | I, W (A) | O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine | non-polymer | 792.1 | 2 | Chemie (P5S) PubChem (9547075) PubChem (10417847) PubChem (9547096) PubChem (125040277) PubChem (15409341) PubChem (133612130) PubChem (165429904) | |||
| 8 | IA, J, K, L, O (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 5 | Chemie (Y01) PubChem (16219150) PubChem (22210610) PubChem (65082) PubChem (363416) PubChem (45489984) PubChem (500055) PubChem (60078761) PubChem (57163942) PubChem (73440805) PubChem (89891013) PubChem (13301926) PubChem (13301927) PubChem (99567469) PubChem (99567468) PubChem (124427194) PubChem (124897021) PubChem (124897020) PubChem (10005657) PubChem (130340716) PubChem (11969853) PubChem (124301053) PubChem (124302591) PubChem (124303794) PubChem (124421343) PubChem (124427195) PubChem (137392566) PubChem (137496323) PubChem (124914595) PubChem (124914596) PubChem (131705526) PubChem (155804219) PubChem (137332121) PubChem (137496357) PubChem (146157332) PubChem (169132514) PubChem (154929912) PubChem (155199678) PubChem (156327905) PubChem (156426247) PubChem (156516588) PubChem (166979870) PubChem (167024614) PubChem (169132513) PubChem (170117245) PubChem (172400028) | |||
| 9 | M (A) | (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en | non-polymer | 544.8 | 1 | Chemie (9Z9) PubChem (134817783) | |||
| 10 | AA, BA, CA, DA, EA... (A, B) | 1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 510.7 | 14 | Chemie (LPE) PubChem (3855) PubChem (9546711) PubChem (446876) | |||
| 11 | GA, HA, S, V, X (A) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 5 | Chemie (PCW) |
Sequence modifications
A: 1 - 1988 (UniProt: Q15858)
| PDB | External Database | Details |
|---|---|---|
| Met -42 | - | expression tag |
| Ala -41 | - | expression tag |
| Ser -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Lys 406 | Glu 406 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 280299.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 17053.4 | |
| All* | Total formula weight | 298626.5 |
