7W2V
Crystal structure of TxGH116 R786A mutant from Thermoanaerobacterium xylanolyticum with glucose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glucosylceramidase | polymer | 842 | 96506.3 | 1 | UniProt (F6BL85) Pfam (PF12215) Pfam (PF04685) | Thermoanaerobacterium xylanolyticum (strain ATCC 49914 / DSM 7097 / LX-11) | |
| 2 | B (A) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 3 | C, D, E, F, G... (A) | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
| 4 | K (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 5 | L (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 6 | M (A) | water | water | 18.0 | 429 | Chemie (HOH) |
Sequence modifications
A: 19 - 806 (UniProt: F6BL85)
| PDB | External Database | Details |
|---|---|---|
| Met -27 | - | initiating methionine |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Val -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Arg -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Met -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Thr -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Ala -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Lys -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Gln 1 | - | expression tag |
| His 2 | - | expression tag |
| Met 3 | - | expression tag |
| Asp 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Pro 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Leu 8 | - | expression tag |
| Gly 9 | - | expression tag |
| Thr 10 | - | expression tag |
| Asp 11 | - | expression tag |
| Asp 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Asp 14 | - | expression tag |
| Lys 15 | - | expression tag |
| Ala 16 | - | expression tag |
| Met 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Ala 786 | Arg 786 | engineered mutation |
| Leu 807 | - | expression tag |
| Glu 808 | - | expression tag |
| His 809 | - | expression tag |
| His 810 | - | expression tag |
| His 811 | - | expression tag |
| His 812 | - | expression tag |
| His 813 | - | expression tag |
| His 814 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 96506.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1019.1 | |
| All* | Total formula weight | 97525.4 |
