7W1M
Cryo-EM structure of human cohesin-CTCF-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Structural maintenance of chromosomes protein 1A | polymer | 1233 | 143485.1 | 1 | UniProt (Q14683) Pfam (PF02463) Pfam (PF06470) | Homo sapiens (Human) | SMC protein 1A,SMC-1-alpha,SMC-1A,Sb1.8 |
| 2 | B (B) | Structural maintenance of chromosomes protein 3 | polymer | 1217 | 141771.6 | 1 | UniProt (Q9UQE7) Pfam (PF02463) Pfam (PF06470) | Homo sapiens (Human) | SMC protein 3,SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP |
| 3 | C (C) | Double-strand-break repair protein rad21 homolog | polymer | 631 | 71556.1 | 1 | UniProt (O60216) Pfam (PF04825) Pfam (PF04824) | Homo sapiens (Human) | hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog |
| 4 | D (D) | Cohesin subunit SA-1 | polymer | 1258 | 144617.0 | 1 | UniProt (Q8WVM7) Pfam (PF08514) Pfam (PF21581) Pfam (PF24571) | Homo sapiens (Human) | SCC3 homolog 1,Stromal antigen 1 |
| 5 | E (E) | Nipped-B-like protein | polymer | 1467 | 167830.5 | 1 | UniProt (Q6KC79) Pfam (PF12765) Pfam (PF12830) | Homo sapiens (Human) | Delangin,SCC2 homolog |
| 6 | F (F) | DNA (118-MER) | polymer | 118 | 36202.3 | 1 | Homo sapiens | ||
| 7 | G (G) | DNA (118-MER) | polymer | 118 | 36605.4 | 1 | Homo sapiens | ||
| 8 | H (H) | Transcriptional repressor CTCF | polymer | 727 | 82928.8 | 1 | UniProt (P49711) Pfam (PF00096) Pfam (PF23611) | Homo sapiens (Human) | 11-zinc finger protein,CCCTC-binding factor,CTCFL paralog |
| 9 | I, K (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 10 | J, L (B) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 2 | Chemie (BEF) | |||
| 11 | M, N, O, P, Q... (H) | ZINC ION | non-polymer | 65.4 | 11 | Chemie (ZN) |
Sequence modifications
C: 1 - 631 (UniProt: O60216)
| PDB | External Database | Details |
|---|---|---|
| Ala 172 | Arg 172 | engineered mutation |
| Ala 279 | Asp 279 | engineered mutation |
| Ala 450 | Arg 450 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 824996.7 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1705.9 | |
| All* | Total formula weight | 826702.6 |
