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7W1M

Cryo-EM structure of human cohesin-CTCF-DNA complex

Functional Information from GO Data
ChainGOidnamespacecontents
A0000070biological_processmitotic sister chromatid segregation
A0000775cellular_componentchromosome, centromeric region
A0000776cellular_componentkinetochore
A0000794cellular_componentcondensed nuclear chromosome
A0003677molecular_functionDNA binding
A0003682molecular_functionchromatin binding
A0003723molecular_functionRNA binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005694cellular_componentchromosome
A0005829cellular_componentcytosol
A0006281biological_processDNA repair
A0007062biological_processsister chromatid cohesion
A0007064biological_processmitotic sister chromatid cohesion
A0008278cellular_componentcohesin complex
A0009314biological_processresponse to radiation
A0016363cellular_componentnuclear matrix
A0016887molecular_functionATP hydrolysis activity
A0030892cellular_componentmitotic cohesin complex
A0030893cellular_componentmeiotic cohesin complex
A0034087biological_processestablishment of mitotic sister chromatid cohesion
A0034089biological_processestablishment of meiotic sister chromatid cohesion
A0035019biological_processsomatic stem cell population maintenance
A0036033molecular_functionmediator complex binding
A0046982molecular_functionprotein heterodimerization activity
A0051276biological_processchromosome organization
A0051301biological_processcell division
A0051321biological_processmeiotic cell cycle
A0072423biological_processresponse to DNA damage checkpoint signaling
A0090307biological_processmitotic spindle assembly
A0097431cellular_componentmitotic spindle pole
B0000278biological_processmitotic cell cycle
B0000775cellular_componentchromosome, centromeric region
B0000785cellular_componentchromatin
B0000800cellular_componentlateral element
B0003682molecular_functionchromatin binding
B0003690molecular_functiondouble-stranded DNA binding
B0003777molecular_functionmicrotubule motor activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005654cellular_componentnucleoplasm
B0005694cellular_componentchromosome
B0005829cellular_componentcytosol
B0006275biological_processregulation of DNA replication
B0006281biological_processDNA repair
B0007062biological_processsister chromatid cohesion
B0007064biological_processmitotic sister chromatid cohesion
B0008278cellular_componentcohesin complex
B0016363cellular_componentnuclear matrix
B0016887molecular_functionATP hydrolysis activity
B0019827biological_processstem cell population maintenance
B0030892cellular_componentmitotic cohesin complex
B0030893cellular_componentmeiotic cohesin complex
B0034087biological_processestablishment of mitotic sister chromatid cohesion
B0034089biological_processestablishment of meiotic sister chromatid cohesion
B0036033molecular_functionmediator complex binding
B0046982molecular_functionprotein heterodimerization activity
B0048487molecular_functionbeta-tubulin binding
B0051276biological_processchromosome organization
B0051301biological_processcell division
B0051321biological_processmeiotic cell cycle
B0070840molecular_functiondynein complex binding
B0090307biological_processmitotic spindle assembly
B0097431cellular_componentmitotic spindle pole
C0000775cellular_componentchromosome, centromeric region
C0000785cellular_componentchromatin
C0000794cellular_componentcondensed nuclear chromosome
C0000922cellular_componentspindle pole
C0001666biological_processresponse to hypoxia
C0003677molecular_functionDNA binding
C0003682molecular_functionchromatin binding
C0005515molecular_functionprotein binding
C0005634cellular_componentnucleus
C0005654cellular_componentnucleoplasm
C0005694cellular_componentchromosome
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0005856cellular_componentcytoskeleton
C0006281biological_processDNA repair
C0006302biological_processdouble-strand break repair
C0006310biological_processDNA recombination
C0006357biological_processregulation of transcription by RNA polymerase II
C0006915biological_processapoptotic process
C0007059biological_processchromosome segregation
C0007062biological_processsister chromatid cohesion
C0007131biological_processreciprocal meiotic recombination
C0008278cellular_componentcohesin complex
C0010628biological_processpositive regulation of gene expression
C0010629biological_processnegative regulation of gene expression
C0010972biological_processnegative regulation of G2/M transition of mitotic cell cycle
C0016020cellular_componentmembrane
C0016363cellular_componentnuclear matrix
C0030496cellular_componentmidbody
C0030892cellular_componentmitotic cohesin complex
C0030893cellular_componentmeiotic cohesin complex
C0032691biological_processnegative regulation of interleukin-1 beta production
C0032720biological_processnegative regulation of tumor necrosis factor production
C0032733biological_processpositive regulation of interleukin-10 production
C0034087biological_processestablishment of mitotic sister chromatid cohesion
C0034089biological_processestablishment of meiotic sister chromatid cohesion
C0034351biological_processnegative regulation of glial cell apoptotic process
C0043524biological_processnegative regulation of neuron apoptotic process
C0045841biological_processnegative regulation of mitotic metaphase/anaphase transition
C0045876biological_processpositive regulation of sister chromatid cohesion
C0051301biological_processcell division
C0071168biological_processprotein localization to chromatin
C0106222molecular_functionlncRNA binding
C0140297molecular_functionDNA-binding transcription factor binding
C0140588biological_processchromatin looping
C1990414biological_processreplication-born double-strand break repair via sister chromatid exchange
D0000775cellular_componentchromosome, centromeric region
D0000785cellular_componentchromatin
D0003682molecular_functionchromatin binding
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005694cellular_componentchromosome
D0005829cellular_componentcytosol
D0007059biological_processchromosome segregation
D0007062biological_processsister chromatid cohesion
D0008278cellular_componentcohesin complex
D0016363cellular_componentnuclear matrix
D0016604cellular_componentnuclear body
D0030892cellular_componentmitotic cohesin complex
D0034087biological_processestablishment of mitotic sister chromatid cohesion
D0051301biological_processcell division
D0090307biological_processmitotic spindle assembly
D0097431cellular_componentmitotic spindle pole
E0003682molecular_functionchromatin binding
E0010468biological_processregulation of gene expression
E0061780biological_processmitotic cohesin loading
H0000122biological_processnegative regulation of transcription by RNA polymerase II
H0000775cellular_componentchromosome, centromeric region
H0000793cellular_componentcondensed chromosome
H0000976molecular_functiontranscription cis-regulatory region binding
H0000978molecular_functionRNA polymerase II cis-regulatory region sequence-specific DNA binding
H0001221molecular_functiontranscription coregulator binding
H0001227molecular_functionDNA-binding transcription repressor activity, RNA polymerase II-specific
H0001673cellular_componentmale germ cell nucleus
H0001701biological_processin utero embryonic development
H0003677molecular_functionDNA binding
H0003682molecular_functionchromatin binding
H0003700molecular_functionDNA-binding transcription factor activity
H0005515molecular_functionprotein binding
H0005634cellular_componentnucleus
H0005654cellular_componentnucleoplasm
H0005694cellular_componentchromosome
H0005730cellular_componentnucleolus
H0006325biological_processchromatin organization
H0006346biological_processDNA methylation-dependent heterochromatin formation
H0006355biological_processregulation of DNA-templated transcription
H0007005biological_processmitochondrion organization
H0007059biological_processchromosome segregation
H0007507biological_processheart development
H0008270molecular_functionzinc ion binding
H0008285biological_processnegative regulation of cell population proliferation
H0010467biological_processgene expression
H0010628biological_processpositive regulation of gene expression
H0010629biological_processnegative regulation of gene expression
H0040029biological_processepigenetic regulation of gene expression
H0043035molecular_functionchromatin insulator sequence binding
H0043565molecular_functionsequence-specific DNA binding
H0044027biological_processnegative regulation of gene expression via chromosomal CpG island methylation
H0045892biological_processnegative regulation of DNA-templated transcription
H0045893biological_processpositive regulation of DNA-templated transcription
H0045944biological_processpositive regulation of transcription by RNA polymerase II
H0046872molecular_functionmetal ion binding
H0055007biological_processcardiac muscle cell differentiation
H0055013biological_processcardiac muscle cell development
H0070602biological_processregulation of centromeric sister chromatid cohesion
H0071459biological_processprotein localization to chromosome, centromeric region
H0071514biological_processgenomic imprinting
H0140587molecular_functionchromatin loop anchoring activity
H0140588biological_processchromatin looping
H1990837molecular_functionsequence-specific double-stranded DNA binding
Functional Information from PROSITE/UniProt
site_idPS00028
Number of Residues21
DetailsZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cel..CsytCprrsnldrHmks..H
ChainResidueDetails
HCYS268-HIS288
HCYS296-HIS316
HCYS324-HIS345
HCYS409-HIS430
HCYS439-HIS460
HCYS469-HIS489
HCYS497-HIS517
HCYS525-HIS546

site_idPS00211
Number of Residues15
DetailsABC_TRANSPORTER_1 ABC transporters family signature. LSGGQKSLVALALIF
ChainResidueDetails
BLEU1115-PHE1129
ALEU1128-PHE1142

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues22
DetailsZN_FING: C2H2-type 1 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE266-HIS288
CALA450

site_idSWS_FT_FI2
Number of Residues22
DetailsZN_FING: C2H2-type 2 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HHIS294-HIS316
DSER1093

site_idSWS_FT_FI3
Number of Residues23
DetailsZN_FING: C2H2-type 3 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HHIS322-HIS345
BLYS1190

site_idSWS_FT_FI4
Number of Residues22
DetailsZN_FING: C2H2-type 4 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE351-HIS373
ESER2511
ESER2513
ESER2515

site_idSWS_FT_FI5
Number of Residues22
DetailsZN_FING: C2H2-type 5 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE379-HIS401

site_idSWS_FT_FI6
Number of Residues23
DetailsZN_FING: C2H2-type 6 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HTYR407-HIS430

site_idSWS_FT_FI7
Number of Residues23
DetailsZN_FING: C2H2-type 7 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE437-HIS460
CTHR623

site_idSWS_FT_FI8
Number of Residues22
DetailsZN_FING: C2H2-type 8 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HLYS467-HIS489
CSER545

site_idSWS_FT_FI9
Number of Residues22
DetailsZN_FING: C2H2-type 9 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE495-HIS517
CLYS216
CLYS418

site_idSWS_FT_FI10
Number of Residues23
DetailsZN_FING: C2H2-type 10 => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HTYR523-HIS546

site_idSWS_FT_FI11
Number of Residues22
DetailsZN_FING: C2H2-type 11; atypical => ECO:0000255|PROSITE-ProRule:PRU00042
ChainResidueDetails
HPHE555-CYS577

site_idSWS_FT_FI12
Number of Residues1
DetailsMOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378
ChainResidueDetails
HMET1

site_idSWS_FT_FI13
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231
ChainResidueDetails
HTHR289
HTHR317

site_idSWS_FT_FI14
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648
ChainResidueDetails
HTHR374

site_idSWS_FT_FI15
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648
ChainResidueDetails
HSER402

site_idSWS_FT_FI16
Number of Residues3
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692
ChainResidueDetails
HSER609
HSER610
HSER612

site_idSWS_FT_FI17
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733
ChainResidueDetails
HLYS18
HLYS219

site_idSWS_FT_FI18
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:28112733
ChainResidueDetails
HLYS689

site_idSWS_FT_FI19
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) => ECO:0000250
ChainResidueDetails
HLYS74

224004

PDB entries from 2024-08-21

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