7W0N

Cryo-EM structure of a dimeric GPCR-Gi complex with peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	356	40559.2	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	Q, R	Soluble cytochrome b562,Apelin receptor	polymer	477	54312.6	2	UniProt (P0ABE7) UniProt (P35414) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11
5	S	scFv16	polymer	297	31870.6	1		Homo sapiens
6	D, E	Apelin receptor early endogenous ligand	polymer	32	3980.9	2	UniProt (P0DMC3) Pfam (PF22050) In PDB	Homo sapiens	Protein Elabela,ELA,Protein Toddler

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Q, R: -104 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -146	-	initiating methionine
Lys -145	-	expression tag
Thr -144	-	expression tag
Ile -143	-	expression tag
Ile -142	-	expression tag
Ala -141	-	expression tag
Leu -140	-	expression tag
Ser -139	-	expression tag
Tyr -138	-	expression tag
Ile -137	-	expression tag
Phe -136	-	expression tag
Cys -135	-	expression tag
Leu -134	-	expression tag
Val -133	-	expression tag
Phe -132	-	expression tag
Ala -131	-	expression tag
Asp -130	-	expression tag
Tyr -129	-	expression tag
Lys -128	-	expression tag
Asp -127	-	expression tag
Asp -126	-	expression tag
Asp -125	-	expression tag
Asp -124	-	expression tag
Lys -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
Leu -112	-	expression tag
Glu -111	-	expression tag
Val -110	-	expression tag
Leu -109	-	expression tag
Phe -108	-	expression tag
Gln -107	-	expression tag
Gly -106	-	expression tag
Pro -105	-	expression tag
Trp -98	Met 29	conflict
Ile -3	His 124	conflict
Leu 1	-	linker

Q, R: 2 - 330 (UniProt: P35414)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	234294.8
All*	Total formula weight	234294.8

*Water molecules are not included.