7W05
12 mutant Ribonuclease from Hericium erinaceus GMP binding form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribonuclease T1 | polymer | 100 | 10703.5 | 1 | UniProt (B1Q4V2) Pfam (PF00545) | Hericium erinaceus (Lion's mane mushroom Hydnum erinaceus) | |
| 2 | B (A) | GUANOSINE | non-polymer | 283.2 | 1 | Chemie (GMP) | |||
| 3 | C (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 4 | D (A) | water | water | 18.0 | 130 | Chemie (HOH) |
Sequence modifications
A: 1 - 100 (UniProt: B1Q4V2)
| PDB | External Database | Details |
|---|---|---|
| Asn 19 | Asp 43 | engineered mutation |
| Asn 22 | Asp 46 | engineered mutation |
| Gln 25 | Glu 49 | engineered mutation |
| Asn 31 | Asp 55 | engineered mutation |
| Asn 38 | Asp 62 | engineered mutation |
| Gln 50 | Glu 74 | engineered mutation |
| Gln 57 | Glu 81 | engineered mutation |
| Gln 76 | Glu 100 | engineered mutation |
| Asn 77 | Asp 101 | engineered mutation |
| Asn 79 | Asp 103 | engineered mutation |
| Gln 92 | Glu 116 | engineered mutation |
| Asn 93 | Asp 117 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 10703.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 389.4 | |
| All* | Total formula weight | 11092.8 |
