7W05
12 mutant Ribonuclease from Hericium erinaceus GMP binding form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.589, 46.310, 53.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.995 - 1.290 |
R-factor | 0.1382 |
Rwork | 0.137 |
R-free | 0.16620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ls1 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.310 | 1.210 |
High resolution limit [Å] | 1.190 | 1.190 |
Number of reflections | 22027 | 1444 |
<I/σ(I)> | 33.78 | |
Completeness [%] | 98.8 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Cadmium chloride hydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v Polyethylene glycol 400 |