7VUG
Cryo-EM structure of a class A orphan GPCR in complex with Gi
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 353 | 40283.8 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 346 | 38045.6 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
4 | R | Chimera of Endo-1,4-beta-xylanase and Probable G-protein coupled receptor 139 | polymer | 533 | 60890.1 | 1 | UniProt (P09850) UniProt (Q6DWJ6) Pfam (PF00457) Pfam (PF00001) In PDB | Bacillus circulans | Xylanase,1,4-beta-D-xylan xylanohydrolase,G(q)-coupled orphan receptor GPRg1,G-protein-coupled receptor PGR3 |
5 | S | scFv16 | polymer | 259 | 27784.9 | 1 | Homo sapiens | ||
6 | R | 3-chloranyl-N-[2-oxidanylidene-2-[[(1S)-1-phenylethyl]amino]ethyl]benzamide | non-polymer | 316.8 | 1 | Chemie (7ZQ) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: -183 - 1 (UniProt: P09850)
R: 2 - 321 (UniProt: Q6DWJ6)
PDB | External Database | Details |
---|---|---|
Ile -5 | - | expression tag |
Gly -4 | - | expression tag |
Arg -3 | - | expression tag |
Ala -2 | - | expression tag |
Arg -1 | - | expression tag |
Gly 0 | - | expression tag |
Phe 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
His -211 | - | expression tag |
His -210 | - | expression tag |
His -209 | - | expression tag |
His -208 | - | expression tag |
His -207 | - | expression tag |
His -206 | - | expression tag |
His -205 | - | expression tag |
His -204 | - | expression tag |
His -203 | - | expression tag |
His -202 | - | expression tag |
Asp -201 | - | expression tag |
Tyr -200 | - | expression tag |
Lys -199 | - | expression tag |
Asp -198 | - | expression tag |
Asp -197 | - | expression tag |
Asp -196 | - | expression tag |
Asp -195 | - | expression tag |
Lys -194 | - | expression tag |
Glu -193 | - | expression tag |
Asn -192 | - | expression tag |
Leu -191 | - | expression tag |
Tyr -190 | - | expression tag |
Phe -189 | - | expression tag |
Gln -188 | - | expression tag |
Ser -187 | - | expression tag |
Gly -186 | - | expression tag |
Ala -185 | - | expression tag |
Pro -184 | - | expression tag |
Phe -173 | Asp 39 | engineered mutation |
Asp -62 | Arg 150 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Val 62 | Ser 62 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 174865.6 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 316.8 | |
All* | Total formula weight | 175182.4 |