7VUG

Cryo-EM structure of a class A orphan GPCR in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	353	40283.8	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (R)	Chimera of Endo-1,4-beta-xylanase and Probable G-protein coupled receptor 139	polymer	533	60890.1	1	UniProt (P09850) UniProt (Q6DWJ6) Pfam (PF00457) Pfam (PF00001)	Bacillus circulans	Xylanase,1,4-beta-D-xylan xylanohydrolase,G(q)-coupled orphan receptor GPRg1,G-protein-coupled receptor PGR3
5	E (S)	scFv16	polymer	259	27784.9	1		Homo sapiens
6	F (R)	3-chloranyl-N-[2-oxidanylidene-2-[[(1S)-1-phenylethyl]amino]ethyl]benzamide	non-polymer		316.8	1	Chemie (7ZQ)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: -183 - 1 (UniProt: P09850)

PDB	External Database	Details
His -211	-	expression tag
His -210	-	expression tag
His -209	-	expression tag
His -208	-	expression tag
His -207	-	expression tag
His -206	-	expression tag
His -205	-	expression tag
His -204	-	expression tag
His -203	-	expression tag
His -202	-	expression tag
Asp -201	-	expression tag
Tyr -200	-	expression tag
Lys -199	-	expression tag
Asp -198	-	expression tag
Asp -197	-	expression tag
Asp -196	-	expression tag
Asp -195	-	expression tag
Lys -194	-	expression tag
Glu -193	-	expression tag
Asn -192	-	expression tag
Leu -191	-	expression tag
Tyr -190	-	expression tag
Phe -189	-	expression tag
Gln -188	-	expression tag
Ser -187	-	expression tag
Gly -186	-	expression tag
Ala -185	-	expression tag
Pro -184	-	expression tag
Phe -173	Asp 39	engineered mutation
Asp -62	Arg 150	engineered mutation

R: 2 - 321 (UniProt: Q6DWJ6)

PDB	External Database	Details
Val 62	Ser 62	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	174865.6
Non-Polymers*	Number of molecules	1
	Total formula weight	316.8
All*	Total formula weight	175182.4

*Water molecules are not included.