7VQX

Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45743.4	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	400	43706.8	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	L	Pituitary adenylate cyclase-activating polypeptide 27	polymer	27	3154.6	1	UniProt (P18509) Pfam (PF00123) In PDB	Homo sapiens	PACAP-27,PACAP27
5	N	Nanobody-35	polymer	140	15343.0	1		synthetic construct
6	R	Vasoactive intestinal polypeptide receptor 2	polymer	573	64579.4	1	UniProt (P41587) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	VIP-R-2,Helodermin-preferring VIP receptor,Pituitary adenylate cyclase-activating polypeptide type III receptor,PACAP type III receptor,PACAP-R-3,PACAP-R3,VPAC2

Sequence modifications

A: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation
Ser 366	Ala 366	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -33	-	initiating methionine
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 24 - 438 (UniProt: P41587)

PDB	External Database	Details
Val 439	-	expression tag
Phe 440	-	expression tag
Thr 441	-	expression tag
Leu 442	-	expression tag
Glu 443	-	expression tag
Asp 444	-	expression tag
Phe 445	-	expression tag
Val 446	-	expression tag
Gly 447	-	expression tag
Asp 448	-	expression tag
Trp 449	-	expression tag
Glu 450	-	expression tag
Gln 451	-	expression tag
Thr 452	-	expression tag
Ala 453	-	expression tag
Ala 454	-	expression tag
Tyr 455	-	expression tag
Asn 456	-	expression tag
Leu 457	-	expression tag
Asp 458	-	expression tag
Gln 459	-	expression tag
Val 460	-	expression tag
Leu 461	-	expression tag
Glu 462	-	expression tag
Gln 463	-	expression tag
Gly 464	-	expression tag
Gly 465	-	expression tag
Val 466	-	expression tag
Ser 467	-	expression tag
Ser 468	-	expression tag
Leu 469	-	expression tag
Leu 470	-	expression tag
Gln 471	-	expression tag
Asn 472	-	expression tag
Leu 473	-	expression tag
Ala 474	-	expression tag
Val 475	-	expression tag
Ser 476	-	expression tag
Val 477	-	expression tag
Thr 478	-	expression tag
Pro 479	-	expression tag
Ile 480	-	expression tag
Gln 481	-	expression tag
Arg 482	-	expression tag
Ile 483	-	expression tag
Val 484	-	expression tag
Arg 485	-	expression tag
Ser 486	-	expression tag
Gly 487	-	expression tag
Glu 488	-	expression tag
Asn 489	-	expression tag
Ala 490	-	expression tag
Leu 491	-	expression tag
Lys 492	-	expression tag
Ile 493	-	expression tag
Asp 494	-	expression tag
Ile 495	-	expression tag
His 496	-	expression tag
Val 497	-	expression tag
Ile 498	-	expression tag
Ile 499	-	expression tag
Pro 500	-	expression tag
Tyr 501	-	expression tag
Glu 502	-	expression tag
Gly 503	-	expression tag
Leu 504	-	expression tag
Ser 505	-	expression tag
Ala 506	-	expression tag
Asp 507	-	expression tag
Gln 508	-	expression tag
Met 509	-	expression tag
Ala 510	-	expression tag
Gln 511	-	expression tag
Ile 512	-	expression tag
Glu 513	-	expression tag
Glu 514	-	expression tag
Val 515	-	expression tag
Phe 516	-	expression tag
Lys 517	-	expression tag
Val 518	-	expression tag
Val 519	-	expression tag
Tyr 520	-	expression tag
Pro 521	-	expression tag
Val 522	-	expression tag
Asp 523	-	expression tag
Asp 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
Phe 527	-	expression tag
Lys 528	-	expression tag
Val 529	-	expression tag
Ile 530	-	expression tag
Leu 531	-	expression tag
Pro 532	-	expression tag
Tyr 533	-	expression tag
Gly 534	-	expression tag
Thr 535	-	expression tag
Leu 536	-	expression tag
Val 537	-	expression tag
Ile 538	-	expression tag
Asp 539	-	expression tag
Gly 540	-	expression tag
Val 541	-	expression tag
Thr 542	-	expression tag
Pro 543	-	expression tag
Asn 544	-	expression tag
Met 545	-	expression tag
Leu 546	-	expression tag
Asn 547	-	expression tag
Tyr 548	-	expression tag
Phe 549	-	expression tag
Gly 550	-	expression tag
Arg 551	-	expression tag
Pro 552	-	expression tag
Tyr 553	-	expression tag
Glu 554	-	expression tag
Gly 555	-	expression tag
Ile 556	-	expression tag
Ala 557	-	expression tag
Val 558	-	expression tag
Phe 559	-	expression tag
Asp 560	-	expression tag
Gly 561	-	expression tag
Lys 562	-	expression tag
Lys 563	-	expression tag
Ile 564	-	expression tag
Thr 565	-	expression tag
Val 566	-	expression tag
Thr 567	-	expression tag
Gly 568	-	expression tag
Thr 569	-	expression tag
Leu 570	-	expression tag
Trp 571	-	expression tag
Asn 572	-	expression tag
Gly 573	-	expression tag
Asn 574	-	expression tag
Lys 575	-	expression tag
Ile 576	-	expression tag
Ile 577	-	expression tag
Asp 578	-	expression tag
Glu 579	-	expression tag
Arg 580	-	expression tag
Leu 581	-	expression tag
Ile 582	-	expression tag
Thr 583	-	expression tag
Pro 584	-	expression tag
Asp 585	-	expression tag
Gly 586	-	expression tag
Ser 587	-	expression tag
Met 588	-	expression tag
Leu 589	-	expression tag
Phe 590	-	expression tag
Arg 591	-	expression tag
Val 592	-	expression tag
Thr 593	-	expression tag
Ile 594	-	expression tag
Asn 595	-	expression tag
Ser 596	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	180388.4
All*	Total formula weight	180388.4

*Water molecules are not included.