7VPU

Crystal structure of the ligand-binding domain of L. thermotolerans Upc2 in complex with ergosterol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Sterol uptake control protein 2 (Upc2)	polymer	332	37117.3	4	UniProt (C5DKV6) Pfam (PF11951)	Lachancea thermotolerans CBS 6340
2	E, F, G, H (A, B, C, D)	ERGOSTEROL	non-polymer		396.6	4	Chemie (ERG)

Sequence modifications

A, B, C, D: 471 - 788 (UniProt: C5DKV6)

PDB	External Database	Details
Met 445	-	initiating methionine
Ser 446	-	expression tag
Tyr 447	-	expression tag
Tyr 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Asp 455	-	expression tag
Tyr 456	-	expression tag
Asp 457	-	expression tag
Ile 458	-	expression tag
Pro 459	-	expression tag
Thr 460	-	expression tag
Leu 461	-	expression tag
Val 462	-	expression tag
Pro 463	-	expression tag
Arg 464	-	expression tag
Gly 465	-	expression tag
Ser 466	-	expression tag
Ala 467	-	expression tag
Met 468	-	expression tag
Gly 469	-	expression tag
Ser 470	-	expression tag
-	Ser 587	deletion
-	Ser 588	deletion
-	Ser 589	deletion
-	Ala 590	deletion
-	Val 591	deletion
-	Ser 592	deletion
-	Glu 593	deletion
-	Ser 594	deletion
-	Leu 595	deletion
-	Ala 596	deletion
-	Ala 597	deletion
-	Ser 598	deletion
Val 599	Ser 599	engineered mutation
Asp 600	Met 600	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148469.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1586.6
All*	Total formula weight	150056.0

*Water molecules are not included.