7VN6
Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning CGCACGTGCG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | C, D, A, B | Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1 | polymer | 439 | 48419.8 | 4 | UniProt (A0A4P1LXE0) UniProt (Q8S307) Pfam (PF01547) Pfam (PF05687) UniProt (by SIFTS) (P0AEX9) In PDB | Serratia sp. (strain FS14) | Protein BIN2 SUBSTRATE 2 |
| 2 | G, H, E, F | DNA (5'-D(*TP*TP*CP*GP*CP*AP*CP*GP*TP*GP*CP*GP*AP*AP*A)-3') | polymer | 15 | 4594.0 | 4 | synthetic construct | ||
| 3 | I, K, L, M | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
| 4 | C, G, A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 7 | Chemie (EDO) |
Sequence modifications
C, D, A, B: -367 - 0 (UniProt: A0A4P1LXE0)
C, D, A, B: 21 - 90 (UniProt: Q8S307)
| PDB | External Database | Details |
|---|---|---|
| Met -368 | - | initiating methionine |
| Ala -286 | Asp 84 | engineered mutation |
| Ala -285 | Lys 85 | engineered mutation |
| Ala -196 | Glu 174 | engineered mutation |
| Ala -195 | Asn 175 | engineered mutation |
| Ala -129 | Lys 241 | engineered mutation |
| Ala -9 | Glu 361 | engineered mutation |
| Ala -6 | Lys 364 | engineered mutation |
| Ala -5 | Asp 365 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 212055.3 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 434.5 | |
| All* | Total formula weight | 213859.0 |
