7VN6
Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning CGCACGTGCG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.300, 93.608, 112.058 |
Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
Resolution | 42.750 - 2.790 |
R-factor | 0.2182 |
Rwork | 0.216 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zd4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.721 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 2.880 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 51574 | 4479 |
<I/σ(I)> | 99.3 | 1.7 |
Completeness [%] | 99.6 | |
Redundancy | 3.4 | |
CC(1/2) | 0.999 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10% (w/v) polyethylene glycol (PEG) 4000 |