7VGH
Cryo-EM structure of the human P4-type flippase ATP8B1-CDC50B in the auto-inhibited E2P state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Cell cycle control protein 50B | polymer | 363 | 40307.4 | 1 | UniProt (Q3MIR4) Pfam (PF03381) In PDB | Homo sapiens (Human) | P4-ATPase flippase complex beta subunit TMEM30B,Transmembrane protein 30B |
2 | B | Phospholipid-transporting ATPase IC | polymer | 1294 | 148538.5 | 1 | UniProt (O43520) Pfam (PF16209) Pfam (PF00122) Pfam (PF13246) Pfam (PF16212) In PDB | Homo sapiens (Human) | ATPase class I type 8B member 1,Familial intrahepatic cholestasis type 1,P4-ATPase flippase complex alpha subunit ATP8B1 |
3 | C | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G48068RF) | |||
4 | D | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G79306TC) | |||
5 | E | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
6 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
7 | B | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 351 (UniProt: Q3MIR4)
B: 1 - 1251 (UniProt: O43520)
PDB | External Database | Details |
---|---|---|
Met -11 | - | initiating methionine |
Ser -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -42 | - | initiating methionine |
Ala -41 | - | expression tag |
Ser -40 | - | expression tag |
Trp -39 | - | expression tag |
Ser -38 | - | expression tag |
His -37 | - | expression tag |
Pro -36 | - | expression tag |
Gln -35 | - | expression tag |
Phe -34 | - | expression tag |
Glu -33 | - | expression tag |
Lys -32 | - | expression tag |
Gly -31 | - | expression tag |
Gly -30 | - | expression tag |
Gly -29 | - | expression tag |
Ala -28 | - | expression tag |
Arg -27 | - | expression tag |
Gly -26 | - | expression tag |
Gly -25 | - | expression tag |
Ser -24 | - | expression tag |
Gly -23 | - | expression tag |
Gly -22 | - | expression tag |
Gly -21 | - | expression tag |
Ser -20 | - | expression tag |
Trp -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
Pro -16 | - | expression tag |
Gln -15 | - | expression tag |
Phe -14 | - | expression tag |
Glu -13 | - | expression tag |
Lys -12 | - | expression tag |
Gly -11 | - | expression tag |
Phe -10 | - | expression tag |
Asp -9 | - | expression tag |
Tyr -8 | - | expression tag |
Lys -7 | - | expression tag |
Asp -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Lys -2 | - | expression tag |
Gly -1 | - | expression tag |
Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 188845.9 | |
Branched | Number of molecules | 3 |
Total formula weight | 2246.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 245.5 | |
All* | Total formula weight | 191337.5 |