7VDO
Crystal structure of KRED F147L/L153Q/Y190P variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (D, A, B, C) | 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogenase | polymer | 252 | 26726.2 | 4 | UniProt (Q6WVP7) | Lactobacillus kefiri | NADPH dependent R-specific alcohol dehydrogenase,SDR family NAD(P)-dependent oxidoreductase |
| 2 | E, F, O, P, S (D, B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 3 | G, K, Q (D, A, B) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 3 | Chemie (NAP) | |||
| 4 | H, L (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | I, M, R (A, B, C) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 6 | J, N (A, B) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 2 | Chemie (BTB) | |||
| 7 | T, U, V, W (D, A, B, C) | water | water | 18.0 | 735 | Chemie (HOH) |
Sequence modifications
D, A, B, C: 2 - 252 (UniProt: Q6WVP7)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Leu 147 | Phe 147 | engineered mutation |
| Gln 153 | Leu 153 | engineered mutation |
| Pro 190 | Tyr 190 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 106904.9 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 3114.0 | |
| All* | Total formula weight | 110018.9 |
