7VDL

Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with circular cortistatin-14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	S	scFv	polymer	285	30363.0	1		Homo sapiens (Human)
5	R	Mas-related G-protein coupled receptor member X2	polymer	330	37124.0	1	UniProt (Q96LB1) Pfam (PF00001) In PDB	Homo sapiens (Human)
6	L	circular cortistatin-14	polymer	14	1726.0	1	UniProt (O00230) Pfam (PF03002) In PDB	Homo sapiens (Human)
7	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

L: 21 - 34 (UniProt: O00230)

PDB	External Database	Details
Lys 23	Arg 94	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	155421.5
Non-Polymers*	Number of molecules	1
	Total formula weight	386.7
All*	Total formula weight	155808.2

*Water molecules are not included.