7V9O

Crystal structure of the lanthipeptide zinc-metallopeptidase EryP mutant E802R from saccharopolyspora erythraea

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alanine aminopeptidase	polymer	884	98262.6	1	UniProt (A4F9D7) Pfam (PF17900) Pfam (PF01433) Pfam (PF11838) In PDB	Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)	Aminopeptidase N,Lysyl aminopeptidase
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4		water	water		18.0	1252	Chemie (HOH)

Sequence modifications

A: 2 - 860 (UniProt: A4F9D7)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Val 1	-	expression tag
Arg 802	Glu 802	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	98262.6
Non-Polymers*	Number of molecules	2
	Total formula weight	260.6
All*	Total formula weight	98523.2

*Water molecules are not included.