7V9O
Crystal structure of the lanthipeptide zinc-metallopeptidase EryP mutant E802R from saccharopolyspora erythraea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.909, 143.583, 60.580 |
Unit cell angles | 90.00, 114.76, 90.00 |
Refinement procedure
Resolution | 19.570 - 1.770 |
R-factor | 0.1397 |
Rwork | 0.139 |
R-free | 0.17060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v9n |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.860 | 19.860 | 1.800 |
High resolution limit [Å] | 1.770 | 9.530 | 1.770 |
Rmerge | 0.079 | 0.040 | 0.322 |
Rmeas | 0.085 | 0.043 | 0.351 |
Rpim | 0.032 | 0.016 | 0.138 |
Total number of observations | 597759 | 3826 | 28121 |
Number of reflections | 88457 | 529 | 4499 |
<I/σ(I)> | 16.1 | 35.6 | 5 |
Completeness [%] | 99.7 | 89.5 | 99.8 |
Redundancy | 6.8 | 7.2 | 6.3 |
CC(1/2) | 0.998 | 0.999 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 20%(w/v), PEG 6000, MES 6.0, 200mM Ammonium chloride |