7V6Y

Cryo-EM structure of Patched in lipid nanodisc - the wildtype, 3.5 angstrom (re-processed with dataset of 7dzq)

Replaces:  7DZQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein patched homolog 1,Protein patched homolog 1	polymer	1090	121637.6	1	UniProt (Q61115) Pfam (PF12349) Pfam (PF02460) In PDB	Mus musculus (Mouse)	PTC,PTC1,PTC,PTC1
2	B	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	A	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
4	A	(2S)-2-azanyl-3-[[(2S)-3-butanoyloxy-2-dec-9-enoyloxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid	non-polymer		481.5	1	Chemie (5VI)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 2 - 709 (UniProt: Q61115)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag

A: 710 - 1175 (UniProt: Q61115)

PDB	External Database	Details
Thr 1176	-	expression tag
Leu 1177	-	expression tag
Glu 1178	-	expression tag
Val 1179	-	expression tag
Leu 1180	-	expression tag
Phe 1181	-	expression tag
Gln 1182	-	expression tag
Gly 1183	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	121637.6
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1918.4
All*	Total formula weight	123980.4

*Water molecules are not included.