7V4P

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-hydroxylase	polymer	197	22717.6	2	UniProt (C4NCJ7) Pfam (PF05118) In PDB	Streptomyces sp. MK730-62F2
2	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3		water	water		18.0	112	Chemie (HOH)

Sequence modifications

A, B: 1 - 182 (UniProt: C4NCJ7)

PDB	External Database	Details
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 117	Asp 117	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45435.1
Non-Polymers*	Number of molecules	2
	Total formula weight	111.7
All*	Total formula weight	45546.8

*Water molecules are not included.