7V4P
Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2020-11-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.083, 82.083, 104.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.460 - 1.950 |
R-factor | 0.1917 |
Rwork | 0.188 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p7x |
RMSD bond length | 0.011 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.460 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 26710 | 26733 |
<I/σ(I)> | 36.9 | |
Completeness [%] | 100.0 | |
Redundancy | 15.7 | |
CC(1/2) | 0.939 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Sodium acetate 0.1M Bis Tris propane pH 6.5 20 % W/V PEG 3350 |