7UXH

cryo-EM structure of the mTORC1-TFEB-Rag-Ragulator complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Serine/threonine-protein kinase mTOR	polymer	2549	289258.0	2	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260)	Homo sapiens (human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1
2	B, D (B, D)	Target of rapamycin complex subunit LST8	polymer	326	35910.1	2	UniProt (Q9BVC4) Pfam (PF00400)	Homo sapiens (human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	E, U (E, U)	Regulatory-associated protein of mTOR	polymer	1335	149200.0	2	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400)	Homo sapiens (human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein
4	CA, F, M, V (F, M, V, c)	Ras-related GTP-binding protein A	polymer	313	36600.2	4	UniProt (Q7L523) Pfam (PF04670)	Homo sapiens (human)	Rag A,RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
5	DA, G, N, W (G, N, W, d)	Ras-related GTP-binding protein C	polymer	399	44298.9	4	UniProt (Q9HB90) Pfam (PF04670)	Homo sapiens (human)	RagC,GTPase-interacting protein 2,TIB929
6	EA, H, O, X (H, O, X, e)	Ragulator complex protein LAMTOR1	polymer	161	17762.8	4	UniProt (Q6IAA8) Pfam (PF15454)	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho
7	FA, I, P, Y (I, P, Y, f)	Ragulator complex protein LAMTOR2	polymer	125	13517.5	4	UniProt (Q9Y2Q5) Pfam (PF03259)	Homo sapiens (human)	Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3
8	GA, J, Q, Z (J, Q, Z, g)	Ragulator complex protein LAMTOR3	polymer	124	13637.7	4	UniProt (Q9UHA4) Pfam (PF08923)	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1
9	AA, HA, K, R (K, R, a, h)	Ragulator complex protein LAMTOR4	polymer	99	10753.2	4	UniProt (Q0VGL1)	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4
10	BA, IA, L, S (L, S, b, i)	Ragulator complex protein LAMTOR5	polymer	91	9622.9	4	UniProt (O43504) Pfam (PF16672)	Homo sapiens (human)	Hepatitis B virus X-interacting protein,HBX-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5
11	JA, T (T, j)	Transcription factor EB	polymer	476	52926.6	2	UniProt (P19484) Pfam (PF15951) Pfam (PF00010) Pfam (PF11851)	Homo sapiens (human)	Class E basic helix-loop-helix protein 35,bHLHe35
12	KA, LA (A, C)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	2	Chemie (IHP)
13	MA, PA, SA, VA (F, M, V, c)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
14	NA, QA, TA, WA (F, M, V, c)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
15	OA, RA, UA, XA (G, N, W, d)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)

Sequence modifications

F, M, V, c: 1 - 313 (UniProt: Q7L523)

PDB	External Database	Details
Leu 66	Gln 66	conflict

G, N, W, d: 1 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Asn 75	Ser 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	36
	Total formula weight	1639361.8
Non-Polymers*	Number of molecules	14
	Total formula weight	5282.8
All*	Total formula weight	1644644.6

*Water molecules are not included.