7UV9
KDM2A-nucleosome structure stabilized by H3K36C-UNC8015 covalent conjugate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3.2 | polymer | 135 | 15231.8 | 2 | UniProt (Q71DI3) Pfam (PF00125) | Homo sapiens (human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (human) | |
| 3 | C, G (C, G) | Histone H2A type 1 | polymer | 129 | 13990.3 | 2 | UniProt (P0C0S8) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | H2A.1,Histone H2A/ptl |
| 4 | D, H (D, H) | Histone H2B type 1-C/E/F/G/I | polymer | 125 | 13806.0 | 2 | UniProt (P62807) Pfam (PF00125) | Homo sapiens (human) | Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l |
| 5 | I (I) | DNA (185-MER) | polymer | 185 | 57400.6 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (185-MER) | polymer | 185 | 56831.2 | 1 | synthetic construct | ||
| 7 | K (K) | Lysine-specific demethylase 2A | polymer | 692 | 79868.1 | 1 | UniProt (Q9Y2K7) Pfam (PF17811) Pfam (PF02008) Pfam (PF16866) | Homo sapiens (human) | CXXC-type zinc finger protein 8,F-box and leucine-rich repeat protein 11,F-box protein FBL7,F-box protein Lilina,F-box/LRR-repeat protein 11,JmjC domain-containing histone demethylation protein 1A,[Histone-H3]-lysine-36 demethylase 1A |
| 8 | L (K) | N-heptanoyl-N-hydroxy-beta-alanine | non-polymer | 217.3 | 1 | Chemie (OH0) | |||
| 9 | M (K) | FE (III) ION | non-polymer | 55.8 | 1 | Chemie (FE) |
Sequence modifications
A, E: 1 - 135 (UniProt: Q71DI3)
K: 2 - 685 (UniProt: Q9Y2K7)
| PDB | External Database | Details |
|---|---|---|
| Cys 36 | Lys 37 | engineered mutation |
| Ala 110 | Cys 111 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| His 686 | - | expression tag |
| His 687 | - | expression tag |
| His 688 | - | expression tag |
| His 689 | - | expression tag |
| His 690 | - | expression tag |
| His 691 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 302682.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 273.1 | |
| All* | Total formula weight | 302955.7 |
